Szatmári, IstvánAgyare, Eunice2019-06-212019-06-212019-06-13http://hdl.handle.net/2437/269914The major goal of this study is to differentiate mouse ESCs into neural progenitor cells (NPCs) and investigate the inducibility of a reporter transgene during this neuronal differentiation. I observed that this particular EGFP (enhanced green fluorescence protein) carrying ESC clone (C8) exhibited a very strong transgene inducibility comparing with the other EGFP clones and also this cell clone retained its transgene inducibility upon neural differentiation: 98% of the drug-induced cells were GFP positive after 6 day of differentiation. In addition, I observed that these ESC derived differentiated cells were also able to form neuronal networks and they expressed neural specific genes (Pax6 and Neurog2).33enBMP Bone Morphogenetic ProteinsbFGF Basic Fibroblast Growth FactorCNS Central Nervous SystemDOX DoxycyclineDMEM Dulbecco's Modified Eagle MediumDMSO Dimethyl SulfoxideEGFP Green Fluorescence ProteinESCs Embryonic Stem cellsFACS Fluorescence Activated Cell SortingFBS Fetal Bovine SerumFGF Fibroblast Growth FactorGFAP Glial Fibrillary Acidic ProteinG418 GeneticHSCs Hematopoietic Stem CellshESCs Human Embryonic Stem CellsICM Inner Cell MassiPSCs Induced Pluripotent Stem CellsIKMC International Knockout Mouse ConsortiumKSR Knockout Serum ReplacementLIF Leukemia Inhibitory FactormESCs Mouse Embryonic Stem CellsMEF Mouse Embryonic FibroblastN2B27 Neurobasal plus B27 supplementNPCs Neuro Progenitor CellsPBS Phosphate Buffered SalineQ- PCR Quantitative Polymerase Chain ReactionRGPs Radial Glial ProgenitorTA TransactivatorDEENK Témalista::Biológiai tudományok