Markovics, ArnoldTóth, Kinga FanniSós, Katalin EszterMagi, JózsefGyöngyösi, AdriennBenyó, ZoltánZouboulis, Christos C.Bíró, TamásOláh, AttilaOláh Attila (1984-) (élettanász)Biró Tamás (1968-) (élettanász)Tóth Kinga Fanni (1992-) (Molekuláris biológus)Markovics Arnold (1988-) (molekuláris biológus)Gyöngyösi Adrienn (1982-) (biológus)2019-07-052019-07-05Journal Of Cellular And Molecular Medicine. -[Epub] : - (2019), p. -. -J. Cell. Mol. Med. -1582-18381582-1838http://hdl.handle.net/2437/270273Nicotinic acid (NA) activates hydroxycarboxylic acid receptor 2 (HCA2), and it is widely used in treating dyslipidemias. Since its side effects include skin dryness, whereas its deficiency can be accompanied by dyssebacia, characterized by sebaceous gland enlargement, we asked if HCA2 is expressed on human sebocytes, and if NA influences sebocyte functions. By using human immortalized SZ95 sebocytes, we found that non-cytotoxic (≤100 μM; MTT-assay) concentrations of NA had no effect on the homeostatic sebaceous lipogenesis (SLG; Nile Red), but normalized excessive, acne-mimicking SLG induced by several lipogenic agents (arachidonic acid, anandamide, linoleic acid+testosterone; Nile Red; 48-hr treatments). Moreover, it exerted significant anti-proliferative actions (CyQUANT-assay), and increased [Ca2+]IC (Fluo-4 AM-based Ca2+-measurement). Although NA did not prevent the lipopolysaccharide-induced pro-inflammatory response (up-regulation [Q-PCR] and release [ELISA] of several pro-inflammatory cytokines) of the sebocytes, collectively, these data support the concept that NA may be effective in suppressing sebum production in vivo. While exploring the mechanism of the sebostatic actions, we found that sebocytes express HCA2 (Q-PCR, immunofluorescent labeling), siRNA-mediated silencing of which prevented the NA-induced Ca2+-signal and the lipostatic action. Collectively, our data introduce NA, and HCA2 activators in general, as novel, potent, and most likely safe sebostatic agents, with possible anti-acne potential.idegen nyelvű folyóiratközlemény külföldi lapbanacnehydroxycarboxylic acid receptor 2nicotinic acidsebaceous lipogenesissebocyteseborrheafolyóiratcikkopen access journalhttp://webpac.lib.unideb.hu:8082/ebib/CorvinaWeb?action=cclfind&resultview=long&ccltext=idno+BIBFORM079699https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.14505szerző10.1111/jcmm.145052019-07-05