2021-06-282021-06-28http://hdl.handle.net/2437/314368Fruit setting behaviour of fruit trees remains to be in the focus of plant breeders and growers. Realizing that most species (cherry, apple, pear etc.) are self-incompatible and certain cultivars are cross-incompatible, mutual fertility properties and their reliable determination are of great interest. This review gives a comprehensive description of all known S-genotyping procedures, i.e. the classical fruit set analysis after open field test crosses; pollen tube growth monitoring with fluorescent microscopy; stylar ribonuclease electrophoresis (using different types of isoelectric focusing and 2-dimension polyacrilamide gel electrophoresis); as well as the most recent polymerase chain reaction based DNA-level analyses and DNA sequencing. The review presented not only gives a compilation of the bases of the methods described but also provides a critical evaluation and a comparative characterization of their applicability.application/pdfCopyright (c) 2018 International Journal of Horticultural ScienceF-boxNEpHGEPCRRosaceaeself-incompatibilityS-genotypeS-ribonucleaseA critical evaluation of methods used for S-genotyping: from trees to DNA levelinfo:eu-repo/semantics/article