Nygård, YvonneKaraffa, LeventeMózsik, László2016-05-052016-05-052016http://hdl.handle.net/2437/226524The filamentous fungus Penicillium chrysogenum is the major producer of the beta-lactam antibiotic penicillin. In addition to the penicillin biosynthesis cluster, the genome of P. chrysogenum contains a large number of uncharacterized secondary metabolite gene clusters. However, many of these clusters are not expressed under laboratory conditions. This thesis summarizes the steps of developing a novel, orthogonal, synthetic transcriptional regulation system in P. chrysogenum. The system utilizes transcriptional regulatory genes to express the gene of interests with a strong but controllable manner. The build system can be used for heterologous expression or silenced genes, even whole gene clusters can be activated with it.54enheterologous expressionsynthetic biologyreporter expressiongene regulationtranscriptional factorsilent gene cluster activationDEENK Témalista::Biológiai tudományok