This research explored the effects of caffeine on human physiology and examined its many origins via the use of High-Performance Liquid Chromatography (HPLC). The content of caffeine in various samples were precisely determined using HPLC. The findings revealed large variations in the caffeine composition across different product. The physiological impacts of caffeine was spoken about as well as caffeine alternatives and much more .The research showed which the samples had the highest caffeine, as seen by the HPLC analysis. The study offers significant knowledge on the origins of caffeine and its possible impacts on the human body.