In my research, I evaluated the impact of Negative Energy Balance in late pregnant and lactating ewes. Body Condition Score , β-Hydroxybutyrate , Non-Esterified Fatty Acids , and glucose emerged as pivotal indicators of Negative Energy Balance and related disorders like ketosis and pregnancy toxemia. Notably, different breeds displayed varied susceptibilities, underscoring the need for breed-specific care. Regular monitoring of these markers can significantly reduce the risks during gestation and lactation. My findings offer a practical approach to manage and preventNEB and NEB-related complications like ketosis and pregnancy toxemia in sheep.