Data integration from multi-omics approaches reveal inflammation dynamics upon muscle tissue injury and regeneration.

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dc.contributor.advisorNagy, Laszlo
dc.contributor.authorGiannakis, Nikolaos
dc.contributor.departmentMolekuláris sejt- és immunbiológia doktori iskolahu
dc.contributor.submitterdepDE--Általános Orvostudományi Kar -- Department of Molecular, Cellular and Immune Biology
dc.date.accessioned2021-01-08T06:57:03Z
dc.date.available2021-01-08T06:57:03Z
dc.date.created2020hu_HU
dc.date.defended2021-01-15
dc.description.abstractSkeletal muscle regeneration upon injury is an active process that is highly orchestrated by immune cells and mechanistically depends on the great level of coordination between biological processes. Indispensable of the regeneration process is the initial inflammation that consists of two phases, initiation and resolution, which are coordinated by metabolites that can mediate it. Lipid mediators have signaling mediated capacities, and here we showed that there is a dynamic regulation of the mediator lipidome, which was consistent in a model of acute sterile injury, and also in a more pathophysiological model upon exercise. We also monitored a marked remodeling of structural lipids, which can act as biosynthetic sources of the lipid mediator pool. Lipid mediators changed dynamically in regard to their biosynthetic sources. We also observed epigenomic alternations during the time course of inflammation. Consistent with these changes was the actively changing transcriptome, which was recapitulated in infiltrating macrophage populations. In conjunction with our observations we asked whether a lipid biomolecule, RvD2, could affect the temporal regulation of macrophage phenotypic switch. Interestingly, we found that RvD2 can facilitate this switch in vivo, enhancing the reparative macrophages populations over their pro-inflammatory counterparts. As a result, through the effector actions of RvD2, regeneration of the injured skeletal muscle tissue was promoted as it was shown through in vivo force measurements in a model of delayed regeneration in mice.hu_HU
dc.description.correctorLB
dc.format.extent101hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/300926
dc.language.isoenhu_HU
dc.subjectmacrophageshu_HU
dc.subjectinflammation dynamics
dc.subjectcardiotoxin
dc.subjecttissue injury
dc.subjectmuscle regeneration
dc.subjecttissue repair
dc.subjectinflammation
dc.subjectresolution
dc.subjectlipid mediator
dc.subjectresolvin D2
dc.subjecttranscriptomic signature
dc.subjectepigenomic landscape
dc.subject.disciplineElméleti orvostudományokhu
dc.subject.sciencefieldOrvostudományokhu
dc.titleData integration from multi-omics approaches reveal inflammation dynamics upon muscle tissue injury and regeneration.hu_HU
dc.title.translatedData integration from multi-omics approaches reveal inflammation dynamics upon muscle tissue injury and regenerationhu_HU
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