Phosphorus detection and quantification in animal feed is crucial for animal growth and the quality and safety of animal products. MP-AES is a cost-effective instrumental technique for this purpose. This study optimized instrumental parameters for the analysis of phosphorus in animal feed using MP-AES, making it a feasible option for chemical industries and domestic laboratories. The developed method was accurate and precise with a recovery percentage of 95%. MP-AES allows for multi-elemental analysis in one go, providing practical advantages for future analytical applications.