Assembly of Markerless CRISPR-Cas9 Deletion Construction in Candida auris

A tétel rövid nézete
dc.contributor.advisorMiklós, Ida
dc.contributor.advisorPapp, László Attila
dc.contributor.authorMunkhtulga, Ujin
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biotechnológiai Intézet
dc.date.accessioned2022-11-11T09:49:03Z
dc.date.available2022-11-11T09:49:03Z
dc.date.created2022-11-09
dc.description.abstractCandida auris is an emerging multi-drug resistant fungal pathogen causing grave concern. In Candida auris, a gene orthologous to the YAP1 gene in Saccharomyces cerevisiae is present. We suspect the YAP1 orthologous gene to have a similar function to that of S. cerevisiae, in which it is responsible for the oxidative stress response. In order to delete the gene and examine its function, a deletion construction employing CRISPR-Cas9 was assembled. CRISPR-Cas9 is a simple and flexible gene editing technology that is capable of cutting DNA at a specific locus. We designed and extracted fragments comprising of the CRISPR component, single guide RNAs, temporary selective marker nourseothricin, and leucine gene to excise the gene.
dc.description.courseBiochemical Engineering
dc.description.degreeBSc/BA
dc.format.extent30
dc.identifier.urihttps://hdl.handle.net/2437/339458
dc.language.isoen
dc.subjectCandida auris
dc.subjectCRISPR-Cas9
dc.subjectDeletion construction
dc.subjectYAP1 gene
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Genetika
dc.subject.dspaceDEENK Témalista::Biológiai tudományok::Mikrobiológia
dc.titleAssembly of Markerless CRISPR-Cas9 Deletion Construction in Candida auris
Fájlok
Gyűjtemények
Theses (Faculty of Science and Technology)