This thesis focused on the fragmentation of biomolecules mainly chromone derivatives using APCI Mass spectrometry. The primary objectives were to analyse the intricate fragmentation pathways of chromone derivatives under Atmospheric Pressure Chemical Ionization (APCI) and to determine the fragmentation stability of these derivatives based on the characteristic collision energy. A thorough evaluation of a set of specific samples was made and sound conclusions were drawn. The research conducted highlights the main findings, implications, and future directions, emphasising the importance of this specialised field of study.