This study revolves around the analysis of PE-type triblock copolymers of polyethylene oxide and polypropylene oxide which are also known as Pluronics. The analysis process begins with the characterization of the Pluronics by MALDI mass spectrometry following by the testing of the antifoaming activity of the Pluronics. Finally, a correlation is devised between the structure and antifoaming activity of the Pluronics.