This study investigated the toxicity of carbon nanodots (CNDs) derived from biomolecular precursors namely glycine, urea, lysine, and citric acid synthesized using thermal processing. The investigation of toxicity of these CNDs samples was done by measuring the foam height progression of the yeast suspensions which is indicative of the model organism's metabolic activity by CO2 production. The statistical models used showed no significant difference on the foam height measurements between the groups at each time point over the course of the whole experiment. However, strong statistical significance on the differences of the measurements between different timepoints under all conditions was observed. The findings suggest that the CNDs have no toxic effect on the model organism since normal yeast foaming activity was still evident upon the addition of the sample CNDs. While this study is limited to short-term exposure, the results could serve as a starting point for further research into the long-term effects of nanomaterials on microbial respiration and possibly beyond that, by beginning an understanding on how nanomaterials and biological systems interact.