Pulsatilla bungeana, an early spring flower species that is found widely in Eurasia shows intraspecific variation that has slightly different characteristics. However, unlike the widespread other variants, P. bungeana var. astragalifolia exhibits distinctive morphological features: purple anthers, broad and simply pinnate leaves, and undivided foliage. Its distribution also narrowed only to the Altai Mountain Regions of Mongolia. Additionally, the positioning of its stamen and styles that differ from the rest of the species, suggest a possible relation with an ancient lineage. This study aims to investigate whether this particular variant is part of P. bungeana or represents a truly different species. The reconstructed phylogenetic analysis using three gene regions (nrITS, accD-psaI, and trnL-F) revealed a clear separation between P. bungeana var. astragalifolia and most P. bungeana samples. The findings strongly suggest its classification as a new distinct species, Pulsatilla astragalifolia.