This thesis talks about the role of Galectin-3 as a potential therapeutic target in cancer. Beginning with a detailed examination of its structural intricacies, the thesis provides a solid foundation for understanding the protein's interactions within the realm of cancer biology. The integration of analytical methods, including X-ray Crystallography and NMR Spectroscopy, adds a layer of depth to our comprehension of Galectin-3's functions. The thesis is transitioned into discussions on ligands and potential diagnostic applications, showcasing a diverse array of compounds and their promising implications.