Hybridization was performed by crossing the two parental strains of Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Interspecific hybrids were produced, and 10 of which were used in this study. Several genetic analyses were done on the Saccharomyces cerevisiae x Saccharomyces kudriavzevii interspecific hybrids to compare their genomic materials as well as their physiological abilities to ferment and assimilate carbohydrates. The interspecific hybrids shows very advantageous characteristics that can be used in the industrial ad biotechnological fields.