Transcription of Ace2 regulated genes in differential ace2 expressing mutants

A tétel rövid nézete
dc.contributor.advisorBatta, Gyula
dc.contributor.authorAysha, Afnan Hossain
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biotechnológiai Intézethu_HU
dc.date.accessioned2021-11-19T07:33:50Z
dc.date.available2021-11-19T07:33:50Z
dc.date.created2021-11-16
dc.description.abstractCell division in Schizosaccharomyces pombe is influenced by ace2 and its associated genes, including the target genes, eng1 and agn1. Since it has a cell cycle typical to most eukaryotes, Sch. pombe was used as a model organism for our study on cell division and septation. The strains of Sch. pombe used in this research were the wild type L-972, ace2Δ mutant, and the transformants “312” (expressing truncated Ace2 protein) and “WT” (expressing the whole Ace2 protein). The experimental steps included isolation of the total RNA, synthesis of cDNA through reverse transcription, performing a quantitative RT-PCR and analyzing the results.hu_HU
dc.description.correctorhbk
dc.description.courseBiochemical Engineeringhu_HU
dc.description.degreeBSc/BAhu_HU
dc.format.extent30hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/325051
dc.language.isoenhu_HU
dc.subjectAce2hu_HU
dc.subjectSchizosaccharomyces pombehu_HU
dc.subjectFission yeasthu_HU
dc.subjectSeptumhu_HU
dc.subjecteng1hu_HU
dc.subjectagn1hu_HU
dc.subjectSeptationhu_HU
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleTranscription of Ace2 regulated genes in differential ace2 expressing mutantshu_HU
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