Development of an ImageJ-Based Method for the Assessment of Diel Activity Patterns in Hydra oligactis: A Comparative Approach with Hydra vulgaris Toward Understanding Light-Driven Rhythms in Early-Diverging Metazoans

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dc.contributor.advisorTökölyi, Jácint
dc.contributor.authorAldonate Pérez, Antonella Florencia
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biológiai és Ökológiai Intézet
dc.date.accessioned2025-06-19T07:12:47Z
dc.date.available2025-06-19T07:12:47Z
dc.date.created2025-05-08
dc.description.abstractThis study aimed to develop a simple and reliable image-based method for quantifying movement in Hydra oligactis and to explore potential diel activity patterns. Using time-lapse recordings and a custom ImageJ macro, movement was measured as changes in %Area across 3000 frames. Image processing involved grayscale conversion, thresholding, and frame subtraction. Temporal trends were visualized using polynomial curve fitting and statistical analysis of six time-based batches. Results showed fluctuating movement patterns, with varying degrees of activity and estimated variability, suggesting non-random behavior that may reflect ultradian rhythms or partial diel regulation. While longer recordings are needed to confirm circadian patterns, this method provides a valuable framework for future studies on behavioral rhythms in early-diverging animals.
dc.description.courseBiology
dc.description.degreeBSc/BA
dc.format.extent30
dc.identifier.urihttps://hdl.handle.net/2437/393412
dc.language.isoen
dc.rights.infoHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectHydra oligactis
dc.subjectmovement analysis
dc.subjectImageJ
dc.subjectdiel activity
dc.subjectultradian rhythm
dc.subjectchronobiology
dc.subject.dspaceBiology
dc.titleDevelopment of an ImageJ-Based Method for the Assessment of Diel Activity Patterns in Hydra oligactis: A Comparative Approach with Hydra vulgaris Toward Understanding Light-Driven Rhythms in Early-Diverging Metazoans
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