Szerző szerinti böngészés "Alrifai Rahaf"

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    Upregulation of inhibitor of DNA binding 1 and 3 is important for efficient thermogenic response in human adipocytes
    Arianti Rini; Vinnai Boglárka Ágnes; Alrifai Rahaf; Karadsheh Gyath; Qais Al-Khafaji Yousif; Póliska Szilárd; Győry Ferenc; Fésüs László; Kristóf Endre; Arianti Rini (1991-) (Biochemist); Vinnai Boglárka Ágnes (1996-) (Molekuláris biológus); Póliska Szilárd (1978-) (molekuláris biológus); Győry Ferenc (1964-) (sebész); Fésüs László (1947-) (orvos biokémikus); Kristóf Endre Károly (1987-) (kutató orvos, sejtbiokémikus); Biokémiai és Molekuláris Biológiai Intézet -- 7; Sebészeti Intézet -- 85; Genomi Medicina és Bioinformatikai Szolgáltató Laboratórium -- 48; ÁOK; Debreceni Egyetem
    Brown and beige adipocytes can be activated by β-adrenergic agonist via cAMP-dependent signaling. Performing RNA-sequencing analysis in human cervical area-derived adipocytes, we found that dibutyryl-cAMP, which can mimic in vivo stimulation of browning and thermogenesis, enhanced the expression of browning and batokine genes and upregulated several signaling pathway genes linked to thermogenesis. We observed that the expression of Inhibitor of DNA binding and cell differentiation (ID) 1 and particularly ID3 was strongly induced by the adrenergic stimulation. The degradation of ID1 and ID3 elicited by the ID antagonist AGX51 during thermogenic activation prevented the induction of proton leak respiration that reflects thermogenesis and abrogated cAMP analogue-stimulated upregulation of thermogenic genes and mitochondrial complex I, II, and IV subunits, independently of the proximal cAMP-PKA signaling pathway. The presented data suggests that ID proteins contribute to efficient thermogenic response of adipocytes during adrenergic stimulation.