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Tétel Szabadon hozzáférhető A Review on Indigenous Goats of East Africa: A Case for Conservation and Management(2024) Kichamu, Nelly; Astuti, Putri Kusuma; Wanjala, George; Strausz, Péter; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető A review on the potential effects of environmental and economic factors on sheep genetic diversity: consequences of climate change(2023) Wanjala, George; Astuti, Putri Kusuma; Bagi, Zoltán; Kichamu, Nelly; Strausz, Péter; Kusza, SzilviaTétel Szabadon hozzáférhető A systematic review on the trend of transcriptomic study in livestock: an effort to unwind the complexity of adaptation in a climate change environment(2025) Astuti, Putri Kusuma; Sárkány, Péter; Wanjala, George; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető An on-station analysis of factors affecting growth traits of Pure Red Maasai and Dorper sheep breeds under an extensive production system(2023) Wanjala, George; Kichamu, Nelly; Cziszter, Ludovic Toma; Astuti, Putri Kusuma; Kusza, SzilviaTétel Szabadon hozzáférhető Assessing Genomic Inbreeding and Homozygosity Patterns in Hungarian Merino Sheep and its Relatives(2023) Wanjala, George; Astuti, Putri Kusuma; Kichamu, Nelly; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető Assessing the genomics structure of Dorper and White Dorper Variants, and Dorper Populations in South Africa and Hungary(2023) Wanjala, George; Astuti, Putri Kusuma; Bagi, Zoltán; Kichamu, Nelly; Strausz, Péter; Kusza, SzilviaTétel Szabadon hozzáférhető Assessing the population structure and genetic variability of Kenyan native goats under extensive production system(2024) Kichamu, Nelly; Wanjala, George; Cziszter, Ludovic Toma; Strausz, Péter; Astuti, Putri Kusuma; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető Buzzing with Intelligence: Current Issues in Apiculture and the Role of Artificial Intelligence (AI) to Tackle It(2024) Astuti, Putri Kusuma; Hegedűs, Bettina; Oleksa, Andrzej; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető Calcium Metabolism, Immunity and Reproduction in Early Postpartum Dairy Cows(2025) Kusza, Szilvia; Bagi, Zoltán; Astuti, Putri Kusuma; Wanjala, George; Szenci, Ottó; Bajcsy, Árpád CsabaTétel Szabadon hozzáférhető Climate change and dairy farming sustainability; a causal loop paradox and its mitigation scenario(2024) Astuti, Putri Kusuma; Ayoob, Afsal; Strausz, Péter; Vakayil, Beena; Kumar, S. Hari; Kusza, SzilviaTétel Szabadon hozzáférhető Continental vs. tropical breed: Immunity comparison under heat stress conditions utilizing qRT-PCR technique(2023) Fajardo, Roland; Astuti, Putri Kusuma; Bagi, Zoltán; Bodrogi, Lilla; Pintér, Tímea; Skoda, Gabriella; Ohran, Husein; Wanjala, George; Kusza, SzilviaTétel Szabadon hozzáférhető Coping with climate change: understanding the genetic makeup for adaptation in sheep under different climate conditions(2025) Astuti, Putri Kusuma; Kusza, Szilvia; Állattenyésztési tudományok doktori iskola; Mezőgazdaság-, Élelmiszertudományi és Környezetgazdálkodási KarThe first research investigated the polymorphism of heat resistance-related genes across several sheep breeds from diverse climatic conditions. A study involving 51 SNPs across 30 genes associated with heat stress was conducted on 720 sheep from 17 distinct breeds acclimated to various temperatures in Hungary, Bosnia and Herzegovina, Morocco, and Romania, utilizing KASP-PCR technology. A total of 32 SNPs (62.74%) from the initial list of 51 SNPs were successfully genotyped in this study; 17 of these were identified as polymorphic (33.33%). All breeds were successful in genotyping for four SNPs: rs161504783-HSP12A, rs588145625-HSPA8, rs588498137-STAT3, and rs602521720-HCRT. Genotype frequency tendency was detected only for the HSPA12A and HSPA8 genes. The Botosani Karakul breed exhibits the highest number of SNPs (4 SNPs) deviating from Hardy-Weinberg equilibrium and has decreased heterozygosity noted in 10 SNPs. PCA failed to distinctly discriminate the breeds, however plot concentration exhibited slight variation among the three groups, with the loading values of HSP90AA1 and IL33 SNPs strongly influencing PC1 (11.21%) and PC2 (9.98%). We validated prior studies indicating that the SNPs of HSPA12A, HSPA8, HSP90AA1, and IL33 are possible candidate markers for thermotolerance adaption in sheep. The second research is a gene expression analysis of seasonal variations in heat-related genes (HSP70, IL10, TLR2, TLR4, and TLR8) across various sheep breeds in Hungary — the indigenous Tsigai, Hungarian Merino, and White Dorper — using qRT-PCR during the peak summer, fall, winter, and spring seasons. The THI on all sampling days remained within the thermoneutral zone, except during the summer season (THI 78.99). Among all genes of interest in this study, the lowest expression relative to the spring season was observed in the autumn. This study observed that in the Hungarian Merino, a cold-tolerant breed highly susceptible to heat stress, the relative gene expression of HSP70 was elevated throughout summer, demonstrating heat-stress-induced HSP70 expression. An overexpression for IL10 has been observed over the summer season for Hungarian Tsigai, but for Hungarian Merino and White Dorper was in winter season. Spring and autumn are seasons when TLR2 and TLR8 gene expression is lower, whereas winter and summer are seasons when relative gene expression is higher. All breeds exhibited overexpression of both genes during the summer. The peak expression of TLR4 was recorded in the summer for Hungarian Merino and White Dorper, but Hungarian indigenous Tsigai exhibited its maximum expression in the winter The indigenous Hungarian Tsigai was clearly the hardiest breed, having developed remarkable thermotolerance and immunity to the harsh Hungarian climate. The third research carried a transcriptome analysis of the skin of Hortobágyi Racka sheep with black (n=4) and white (n=5) coat colors, used high-throughput RNA sequencing, and linked it with thermoregulatory capacity. A total of 5,525,285 reads were sequenced, which were effectively annotated to 21,328 genes from the Ovis aries genome (ARS-UI_Ramb v30). Based on the criteria of FC>1.5 and p-value<0.05, a total of 108 genes demonstrated differential expression between black-coated and white-coated sheep. Of these, 25 genes were identified as downregulated, whilst 83 genes were classified as upregulated. The gene exhibiting the greatest downregulation was KRTAP6-1 (adj.P.Val= 0.192) which a keratin related genes whereas the most significantly elevated genes (adj.P.Val<0.05) included TYRP1, PMEL, TRPM1, MLANA, SLC24A5, SLC24A4, SLC45A2, TYR, IRF4, DCT, OCA2, and DDC, which are genes linked to pigmentation and the biological mechanisms of melanogenesis. The GO and KEGG pathway analysis of DEGs indicated that the majority were linked to oxidative phosphorylation, tyrosine metabolism, metabolic pathways, thermogenesis, and melanogenesis. The protein-protein interactions shows the interaction network associated with pigmentation and melanin synthesis (IRF4, PAX3, SLC24A4, DCT, KIT, TRPM1, MLANA, OCA2, SLC24A5, SLC45A2, TYRP1, and TYR) as well as immune response and inflammation (PECAM1 and GPNMB) ribosomal proteins. The findings indicated that the roles of these pigmentation-related genes in heat stress adaptation occur via pleiotropic effects. This dissertation research has contributed to the understanding of the genetic architecture and molecular basis of adaptive traits in sheep, mediated by complicated mechanisms involving large gene networks in both direct and indirect (pleiotropic) ways.Tétel Szabadon hozzáférhető Genetic diversity and adaptability of native sheep breeds from different climatic zones(2025) Wanjala, George; Bagi, Zoltán; Gavojdian, Dinu; Badaoui, Bouabid; Astuti, Putri Kusuma; Mizeranschi, Alexandru Eugeniu; Ilisiu, Elena; Ohran, Husein; Pasic Juhas, Eva; Loukovitis, Dimitrios; Kawęcka, Aldona; Šveistienẻ, Rūta; Becskei, Zsolt; Strausz, Péter; Kichamu, Nelly; Kusza, SzilviaTétel Szabadon hozzáférhető Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique(2023) Astuti, Putri Kusuma; Gavojdian, Dinu; Ilie, Daniela Elena; Wanjala, George; Monori, István; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető Heat Stress: Can Animals be Stressed but still be Healthy?(2023) Astuti, Putri Kusuma; Fajardo, Roland; Wanjala, George; Kichamu, Nelly; Ohran, Husein; Badaoui, Bouabid; Bagi, Zoltán; Kusza, SzilviaTétel Szabadon hozzáférhető Hungarian indigenous Tsigai, a promising breed for excellent heat tolerance and immunity(2023) Astuti, Putri Kusuma; Bagi, Zoltán; Bodrogi, Lilla; Pintér, Tímea; Skoda, Gabriella; Fajardo, Roland; Kusza, SzilviaTétel Korlátozottan hozzáférhető Hybridization event between wild boars and domestic pigs in HungaryAstuti, Putri Kusuma; Kusza, Szilvia; Mihalik, Bendegúz; DE--Mezőgazdaság- Élelmiszertudományi és Környezetgazdálkodási KarThe hybridization between wild boar and domestic pig has occurred since the first domestication event, and it still occurs until today both naturally or through human intervention. In this study, we used 13 Short Tandem Repeat (STR), 3 W Insertion or Deletion (InDel), and combination of both microsatellite loci to determine the genetic diversity and hybridization occurrence in several sites in Hungary that we divided into three different groupings.The genetic diversity analysis showed that all the population in this study was in deficit of heterozygosity and showed a small genetic distance between each population. However, the GENELAND analysis confirmed the genetic structuring in the populations, but the geographical clustering was not the source of it. The STRUCTURE analysis based on the combination of STR and W InDel markers could successfully provide evidence of the hybridization between domestic pigs and wild boars occurrence in Hungary, and the rate of the hybridization event was lower compared to the overall mean of hybridization occurrence in European Union reported.Tétel Korlátozottan hozzáférhető Identification of candidate coat colour determining genes in Hortobágy Racka sheep using RNA-seqSárkány, Péter; Kusza, Szilvia; Astuti, Putri Kusuma; DE--Természettudományi és Technológiai Kar--Biotechnológiai IntézetDomestic sheep (Ovis aries) have been a part of people’s lives for millennia and are still globally kept as farm animals for their relatively easy keeping and multipurpose utilisation. Unfortunately, due to the increasing challenge in sheep farming, sheep populations are declining globally. In Hungary sheep keeping has a long history and traditional value. Hungary’s most well-known indigenous sheep breed is the Hortobágy Racka sheep. The utilization of Racka sheep for this study was motivated not only by their significance but primarily by the knowledge gaps resulting from the scarcity of research on coat colour genes in these animals. Genes that are related to the regulation of coat colour formation have been studied for many years now, therefore literature on these genes exist, even for some sheep breeds. However, it should be noted that there are a lot of sheep breeds globally, which mean a lot of genetic variations, therefore there is no guarantee that the regulation of wool colour works uniformly among all breeds. Therefore, to broaden our understanding on the genetic architecture of sheep coat colour formation, this present study focuses on identifying genes related to the coat colour formation process in Hortobágy Racka sheep, through RNA sequencing (RNA-seq) and differentially expressed gene (DEG) analysis.Tétel Szabadon hozzáférhető On-station comparative analysis of reproductive and survival performance between Red Maasai, Dorper, and Merino sheep breeds(2023) Wanjala, George; Kichamu, Nelly; Strausz, Péter; Astuti, Putri Kusuma; Kusza, SzilviaTétel Szabadon hozzáférhető Population Genetic Structure of the Wild Boar (Sus scrofa) in the Carpathian Basin(2020) Mihalik, Bendegúz; Frank, Krisztián; Astuti, Putri Kusuma; Szemethy, Dániel; Szendrei, László; Szemethy, László; Kusza, Szilvia; Stéger, Viktor