Szerző szerinti böngészés "Cullere, Marco"

Megjelenítve 1 - 3 (Összesen 3)
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    TételSzabadon hozzáférhető
    Quantitative PCR with 16S rRNA-gene-targeted specific primers for analysis of caecal microbial community in growing rabbits after dietary supplementation of thyme (Thymus vulgaris) and spirulina (Arthrospira platensis)
    Bagóné Vántus, Viola; Dalle Zotte, Antonella; Cullere, Marco; Bónai, András; Dal Bosco, Alessandro; Szendrő, Zsolt; Tornyos, Gábor; Pósa, Roland; Bóta, Brigitta; Kovács, Melinda; Zsolnai, Attila; egyetemi oktató, kutató
    Objective of this study was to evaluate the effect of supplementation of growing rabbits’ diet with 5% spirulina (Arthrospira platensis) and/or 3% (Thymus vulgaris L.), on composition and amount of rabbits caecal microbiota. After weaning, rabbits were randomly sorted to 4 groups (n ¼ 42/group). The control group received a pellet without any supplementation, in treated groups the dietary supplementation was provided until 77 days of age. On 49, 63 and 77 days of age, six healthy animals/group were randomly selected and slaughtered. From the caecal con- tent the amount of total bacteria, Bacteroides, Clostridium leptum and Clostridium coccoides were determined by quantitative polymerase chain reaction (qPCR), with the aid of bacterial ribosome coding DNA. The copy number of total bacteria, C. leptum, C. coccoides and Bacteroides varied between 2.75 1012 to 2.24 1013, 5.25 1011 to 1.82 1012, 2.5 1010 to 6.91 1011 and 5.89 1010 to 1.10 1012, respectively. The amount of investigated bacteria represented 0.6%- 13.4% of the total bacteria. The use of spirulina and/or thyme supplements influenced the amount of the copy number of each bacteria examined between 49 and 77 days of age. Their effect on the total bacteria number was temporary. Spirulina resulted in more Bacteroides on day 63 but significantly lower amount of Clostridia at 63 and at 77 days of age. The antimicro- bial effect of thyme on the absolute Clostridia number was temporary, prevailing on day 63. Thyme resulted in significantly decreasing percentage ratio (within total bacteria) of C. leptum and C. coccoides by 77 days of age
  • Nincs kép
    TételKorlátozottan hozzáférhető
    Quantitative PCR with 16S rRNA-gene-targeted specific primers for analysis of caecal microbial community in growing rabbits after dietary supplementation of thyme (Thymus vulgaris) and spirulina (Arthrospira platensis)
    Bagóné Vántus, Viola; Dalle Zotte, Antonella; Cullere, Marco; Bónai, András; Dal Bosco, Alessandro; Szendrő, Zsolt; Tornyos, Gábor; Pósa, Roland; Bóta, Brigitta; Kovács, Melinda; Zsolnai, Attila; egyetemi oktató, kutató
    Objective of this study was to evaluate the effect of supplementation of growing rabbits’ diet with 5% spirulina (Arthrospira platensis) and/or 3% (Thymus vulgaris L.), on composition and amount of rabbits caecal microbiota. After weaning, rabbits were randomly sorted to 4 groups (n ¼ 42/group). The control group received a pellet without any supplementation, in treated groups the dietary supplementation was provided until 77 days of age. On 49, 63 and 77 days of age, six healthy animals/group were randomly selected and slaughtered. From the caecal content the amount of total bacteria, Bacteroides, Clostridium leptum and Clostridium coccoides were determined by quantitative polymerase chain reaction (qPCR), with the aid of bacterial ribosome coding DNA. The copy number of total bacteria, C. leptum, C. coccoides and Bacteroides varied between 2.75 1012 to 2.24 1013, 5.25 1011 to 1.82 1012, 2.5 1010 to 6.91 1011 and 5.89 1010 to 1.10 1012, respectively. The amount of investigated bacteria represented 0.6%- 13.4% of the total bacteria. The use of spirulina and/or thyme supplements influenced the amount of the copy number of each bacteria examined between 49 and 77 days of age. Their effect on the total bacteria number was temporary. Spirulina resulted in more Bacteroides on day 63 but significantly lower amount of Clostridia at 63 and at 77 days of age. The antimicrobial effect of thyme on the absolute Clostridia number was temporary, prevailing on day 63. Thyme resulted in significantly decreasing percentage ratio (within total bacteria) of C. leptum and C. coccoides by 77 days of age.
  • Nincs kép
    TételSzabadon hozzáférhető
    Rabbit Lines Divergently Selected for Total Body Fat Content: Correlated Responses on Growth Performance and Carcass Traits
    Kasza, Rozália; Donkó, Tamás; Matics, Zsolt; Nagy, István; Csóka, Ádám; Kovács, György; Gerencsér, Zsolt; Dalle Zotte, Antonella; Cullere, Marco; Szendrő, Zsolt; egyetemi oktató, kutató
    The aim of this experiment was to study the effect of divergently selected rabbits for total body fat content (fat index) on growth performance and carcass traits. The fat index was determined at 10 weeks of age by computed tomography and lasted for four consecutive generations. The rabbits with the lowest fat index belonged to the lean line and those of the highest values belonged to the fat line. At generation four, 60 rabbits/line were housed in wire-mesh cages and fed with commercial pellet ad libitum from weaning (5 w of age) to slaughtering (11 w of age). Growth performance, dressing out percentage and carcass adiposity were measured. The lean line showed a better feed conversion ratio (p < 0.001) than the fat line. Furthermore, the carcass of the lean rabbits had the highest proportion of fore (p < 0.020) and hind (p < 0.006) parts. On the contrary, rabbits of the fat line had the highest carcass adiposity (p < 0.001). The divergent selection for total body fat content showed to be effective for both lean and fat lines. Selection for lower total body fat content could be useful for terminal male lines, while the selection for higher total body fat content could be an advantage for rabbit does in providing fat (energy) reserves.