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Tétel Szabadon hozzáférhető Comparison of pens without and with multilevel platforms for growing rabbitsMatics, Zsolt; Farkas, Tamás Péter; Dal Bosco, Alessandro; Szendrő, Zsolt; Filiou, Eirini; Nagy, István; Odermatt, Meinrad; Paci, Gisella; Gerencsér, Zsolt; PhD hallgató; egyetemi oktató, kutatóThis experiment compared the productive performance and location of growing rabbits in pens without and with two-level platforms (wire-mesh or plastic-mesh). A total of 174 rabbits of both sexes weaned at 5 weeks of age were randomly divided into three groups (n ¼ 58 rabbits/group, 2 pens/treatment, 29 rabbits/pen). The floor area of pens was 1.0 1.83 m, and the floor was made of wire-mesh. Two pens were equipped with wire-mesh (WP) and two pens with plastic- mesh elevated platforms (PP) on two levels, and two pens were without platforms (NoP). Treatment had no effect on the productive performance of growing rabbits. Based on video recordings, animal density (rabbits/m2 in each location) was higher (p < .001) on the floor than on the platforms (in WP: 12.0 vs. 5.2, in PP: 10.2 vs. 7.4 rabbits/m2, respectively). Animal density on the floor was higher (p < .001) in front of the platforms than under the platforms (in WP 15.7 vs. 9.8 rabbits/m2 and in PP 13.3 vs. 8.3 rabbits/m2, respectively). The animal density on plat- forms was 1.4 times higher in group of PP than in WP (p < .001). The animal density was 1.6 and 2.9 times higher on the second floor than on the first one (p < .001), in group of PP and WP, respectively. The concentration of cortisol metabolites in faeces and the ratio of injured rabbits were similar in the three groups. The rabbits showed higher preference staying on the floor compared to the platform. Pens with platforms were not influencing productive performance.Tétel Szabadon hozzáférhető Quantitative PCR with 16S rRNA-gene-targeted specific primers for analysis of caecal microbial community in growing rabbits after dietary supplementation of thyme (Thymus vulgaris) and spirulina (Arthrospira platensis)Bagóné Vántus, Viola; Dalle Zotte, Antonella; Cullere, Marco; Bónai, András; Dal Bosco, Alessandro; Szendrő, Zsolt; Tornyos, Gábor; Pósa, Roland; Bóta, Brigitta; Kovács, Melinda; Zsolnai, Attila; egyetemi oktató, kutatóObjective of this study was to evaluate the effect of supplementation of growing rabbits’ diet with 5% spirulina (Arthrospira platensis) and/or 3% (Thymus vulgaris L.), on composition and amount of rabbits caecal microbiota. After weaning, rabbits were randomly sorted to 4 groups (n ¼ 42/group). The control group received a pellet without any supplementation, in treated groups the dietary supplementation was provided until 77 days of age. On 49, 63 and 77 days of age, six healthy animals/group were randomly selected and slaughtered. From the caecal con- tent the amount of total bacteria, Bacteroides, Clostridium leptum and Clostridium coccoides were determined by quantitative polymerase chain reaction (qPCR), with the aid of bacterial ribosome coding DNA. The copy number of total bacteria, C. leptum, C. coccoides and Bacteroides varied between 2.75 1012 to 2.24 1013, 5.25 1011 to 1.82 1012, 2.5 1010 to 6.91 1011 and 5.89 1010 to 1.10 1012, respectively. The amount of investigated bacteria represented 0.6%- 13.4% of the total bacteria. The use of spirulina and/or thyme supplements influenced the amount of the copy number of each bacteria examined between 49 and 77 days of age. Their effect on the total bacteria number was temporary. Spirulina resulted in more Bacteroides on day 63 but significantly lower amount of Clostridia at 63 and at 77 days of age. The antimicro- bial effect of thyme on the absolute Clostridia number was temporary, prevailing on day 63. Thyme resulted in significantly decreasing percentage ratio (within total bacteria) of C. leptum and C. coccoides by 77 days of ageTétel Korlátozottan hozzáférhető Quantitative PCR with 16S rRNA-gene-targeted specific primers for analysis of caecal microbial community in growing rabbits after dietary supplementation of thyme (Thymus vulgaris) and spirulina (Arthrospira platensis)Bagóné Vántus, Viola; Dalle Zotte, Antonella; Cullere, Marco; Bónai, András; Dal Bosco, Alessandro; Szendrő, Zsolt; Tornyos, Gábor; Pósa, Roland; Bóta, Brigitta; Kovács, Melinda; Zsolnai, Attila; egyetemi oktató, kutatóObjective of this study was to evaluate the effect of supplementation of growing rabbits’ diet with 5% spirulina (Arthrospira platensis) and/or 3% (Thymus vulgaris L.), on composition and amount of rabbits caecal microbiota. After weaning, rabbits were randomly sorted to 4 groups (n ¼ 42/group). The control group received a pellet without any supplementation, in treated groups the dietary supplementation was provided until 77 days of age. On 49, 63 and 77 days of age, six healthy animals/group were randomly selected and slaughtered. From the caecal content the amount of total bacteria, Bacteroides, Clostridium leptum and Clostridium coccoides were determined by quantitative polymerase chain reaction (qPCR), with the aid of bacterial ribosome coding DNA. The copy number of total bacteria, C. leptum, C. coccoides and Bacteroides varied between 2.75 1012 to 2.24 1013, 5.25 1011 to 1.82 1012, 2.5 1010 to 6.91 1011 and 5.89 1010 to 1.10 1012, respectively. The amount of investigated bacteria represented 0.6%- 13.4% of the total bacteria. The use of spirulina and/or thyme supplements influenced the amount of the copy number of each bacteria examined between 49 and 77 days of age. Their effect on the total bacteria number was temporary. Spirulina resulted in more Bacteroides on day 63 but significantly lower amount of Clostridia at 63 and at 77 days of age. The antimicrobial effect of thyme on the absolute Clostridia number was temporary, prevailing on day 63. Thyme resulted in significantly decreasing percentage ratio (within total bacteria) of C. leptum and C. coccoides by 77 days of age.