Szerző szerinti böngészés "Markovics Arnold (1988-) (molekuláris biológus)"

Megjelenítve 1 - 2 (Összesen 2)
  • Nincs kép
    TételSzabadon hozzáférhető
    HPLC Analysis of Polyphenols Derived from Hungarian Aszú from Tokaj Wine Region and Its Effect on Inflammation in an In Vitro Model System of Endothelial Cells
    Markovics, Arnold; Csige, László; Szőllősi, Erzsébet; Matyi, Hajnalka; Lukács, Andrea Diána; Perez, Nóra Réka; Bacsó, Zsófia Réka; Stündl, László; Remenyik, Judit; Biró, Attila; Markovics Arnold (1988-) (molekuláris biológus); Szőllősi Erzsébet (1983-) (környezetkutató-ökológus); Matyi Hajnalka (2023-) (xxx); Lukács Andrea Diána (2023-) (xxx); Perez Nóra Réka (2023-) (xxx); Stündl László (1970-) (agrármérnök); Gálné Remenyik Judit (1965-) (vegyész); Bíró Attila (2023-) (xxx); Élelmiszertechnológiai Intézet -- 4061; MÉK; Debreceni Egyetem
    Many studies have been published in recent years regarding the fact that moderate wine consumption, as a part of a balanced diet can have a beneficial effect on human health. The bio- logically active components of wine continue to be the subject of intense research today. In this study, the bioactive molecules of Hungarian aszú from the Tokaj wine region were analyzed using high-performance liquid chromatography (HPLC) and investigated in an in vitro model system of endothelial cells induced by bacterial-derived lipopolysaccharide. The HPLC measurements were performed on a reversed phased column with gradient elution. The non-cytotoxic concentration of the active substance was determined based on 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT)-, apoptosis, and necrosis assays. The antioxidant effect of the extract was determined by evaluating its ability to eliminate ROS. The expressions of the interleukin-(IL)1α, IL1-β, IL-6, and IL-8 pro-inflammatory cytokines and nitric oxide synthase (eNOS) at the mRNA level were evaluated using a quantitative polymerase chain reaction (qPCR). We found that the lipopolysaccharides (LPS)- induced increases in the expressions of the investigated cytokines were significantly suppressed by Hungarian aszú extract, excluding IL-6. In our experimental setup, our treatment had a positive effect on the eNOS expression, which was impaired as a result of the inflammatory manipulation. In our experimental model, the Hungarian aszú extract decreased the LPS-induced increases in the expression of the investigated cytokines and eNOS at the mRNA level, which presumably had a positive effect on the endothelial dysfunction caused by inflammation due to its strong antioxidant and anti-inflammatory effects. Collectively, this research contributes to a more thorough understanding of the bioactive molecules of aszú from the Tokaj wine region.
  • Nincs kép
    TételSzabadon hozzáférhető
    Nicotinic acid suppresses sebaceous lipogenesis of human sebocytes via activating hydroxycarboxylic acid receptor 2 (HCA )
    Markovics, Arnold; Tóth, Kinga Fanni; Sós, Katalin Eszter; Magi, József; Gyöngyösi, Adrienn; Benyó, Zoltán; Zouboulis, Christos C.; Bíró, Tamás; Oláh, Attila; Oláh Attila (1984-) (élettanász); Biró Tamás (1968-) (élettanász); Tóth Kinga Fanni (1992-) (Molekuláris biológus); Markovics Arnold (1988-) (molekuláris biológus); Gyöngyösi Adrienn (1982-) (biológus); Élettani Intézet -- 10; Immunológiai Intézet -- 18; Molekuláris Orvostudomány Doktori Iskola -- 10042; ÁOK; Orvostudományi Doktori Tanács; Debreceni Egyetem
    Nicotinic acid (NA) activates hydroxycarboxylic acid receptor 2 (HCA2), and it is widely used in treating dyslipidemias. Since its side effects include skin dryness, whereas its deficiency can be accompanied by dyssebacia, characterized by sebaceous gland enlargement, we asked if HCA2 is expressed on human sebocytes, and if NA influences sebocyte functions. By using human immortalized SZ95 sebocytes, we found that non-cytotoxic (≤100 μM; MTT-assay) concentrations of NA had no effect on the homeostatic sebaceous lipogenesis (SLG; Nile Red), but normalized excessive, acne-mimicking SLG induced by several lipogenic agents (arachidonic acid, anandamide, linoleic acid+testosterone; Nile Red; 48-hr treatments). Moreover, it exerted significant anti-proliferative actions (CyQUANT-assay), and increased [Ca2+]IC (Fluo-4 AM-based Ca2+-measurement). Although NA did not prevent the lipopolysaccharide-induced pro-inflammatory response (up-regulation [Q-PCR] and release [ELISA] of several pro-inflammatory cytokines) of the sebocytes, collectively, these data support the concept that NA may be effective in suppressing sebum production in vivo. While exploring the mechanism of the sebostatic actions, we found that sebocytes express HCA2 (Q-PCR, immunofluorescent labeling), siRNA-mediated silencing of which prevented the NA-induced Ca2+-signal and the lipostatic action. Collectively, our data introduce NA, and HCA2 activators in general, as novel, potent, and most likely safe sebostatic agents, with possible anti-acne potential.