Szerző szerinti böngészés "Nagy, Peter"

Megjelenítve 1 - 3 (Összesen 3)
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    Cloning of alpha-synuclein for the investigation of its role in Parkinson’s disease
    Bozhinovska, Jovana; Nagy, Peter; Serrano, Gabriela; DE--Természettudományi és Technológiai Kar--Biotechnológiai Intézet
    By implementing the bioinformatics tools for data mining, combined with molecular biology techniques to perform cloning and biophysical imaging techniques, we aim to achieve a more straightforward and advanced imaging method for the aSyn protein. This can be employed to gain deeper insights into the void of Parkinson’s research. We managed to clone the aSyn gene without the bulky GFP molecule into our plasmid of choice, pcDNA6. Furthermore, we completed the primer design as a preparation step for incorporating UAAs and GCE, which can be meaningful for the further aims of the project involving the use of click chemistry reactions. Going back to the core meaning of this research, we were able to take a step forward in imaging the aSyn protein, the leading cause of Parkinson’s disease, at the single-molecule level.
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    TételSzabadon hozzáférhető
    Improved estimation of the ratio of detection efficiencies of excited acceptors and donors for measurements
    Batta, Ágnes; Hajdu, Tímea; Nagy, Peter; Batta Ágnes (2023-) (xxx); Biofizikai és Sejtbiológiai Intézet -- 6; ÁOK; Debreceni Egyetem
    Forster resonance energy transfer (FRET) is a radiationless interaction between a donor and an acceptor whose distance dependence makes it a sensitive tool for studying the oligomerization and the structure of proteins. When FRET is determined by measuring the sensitized emission of the acceptor, a parameter characterizing the ratio of detection efficiencies of an excited acceptor versus an excited donor is invariably involved in the formalism. For FRET measurements involving fluorescent antibodies or other external labels, this parameter, designated by alpha, is usually determined by comparing the intensity of a known number of donors and acceptors in two independent samples leading to a large statistical variability if the sample size is small. Here, we present a method that improves precision by applying microbeads with a calibrated number of antibody binding sites and a donor-acceptor mixture in which donors and acceptors are present in a certain, experimentally determined ratio. A formalism is developed for determining alpha and the superior reproducibility of the proposed method compared to the conventional approach is demonstrated. Since the novel methodology does not require sophisticated calibration samples or special instrumentation, it can be widely applied for the quantification of FRET experiments in biological research.
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    POTENTIAL USES OF BLOCKCHAINS IN HUMAN RESOURCES
    (2023-06-29) Nagy, Peter
    Blockchain technology offers businesses many opportunities for more efficient operation and safer data management. It also becomes easy tostore and share employee data, while the blockchain guarantees that it does not fall into unauthorized hands. The management of financial transactions and the automation of the payment process are also a great advantage for businesses, which can manage the payment of wages andbenefits more efficiently. Another area of application of blockchain technology is the creation of more efficient workflows that can improve productivity and reduce costs. The management of work schedules and optimized work processes will also be easier with the help of the blockchain, so businesses can become more efficient and effective. In this article, the relationship between HR and blockchains was explored through a meta-analysis based on available related publications.