Szerző szerinti böngészés "Picoli, E. A. de T."
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Tétel Szabadon hozzáférhető Organogenesis in eggplant (Solanum melongena L. cv. Embú) as affected by antibiotics and growth regulators(2002) Picoli, E. A. de T.; Cecon, P. R.; Fári, Miklós; Otoni, Wagner CamposTétel Szabadon hozzáférhető Organogenesis in eggplant (Solanum melongena L. cv. Embu) as affected by antibiotics and growth regulators(2002-08-14) Picoli, E. A. de T.; Cecon, P. R.; Fári, M. G.; Otoni, W. C.The influence of antibiotics (cefotaxime, timentin, kanamycin and hygromycin) and growth regulators (indolacetic acid and 6-benzylaminopurine) was evaluated on eggplant organogenesis. Solanum melongena hypocotyl segments (6 to 10 mm length), taken from 16 to 20-days in vitro grown seedlings, were used as explants. The basic medium was composed by MS salts, Gamborg vitamins and 2% sucrose, solidified with agar 0.8% and pH adjusted to 5.7±0.2. Morphogenesis was impaired at 50 to 100 mg L - I kanamycin and 7.5 mg L -1 hygromycin. Both Timentin and cefotaxime reduced the frequency of regenerating explants meanwhile hyperhydricity was not affected. A decrease in root regeneration was observed with increasing cefotaxime concentrations, although, timentin had no effect on root regeneration, as compared to the control treatment. Interestingly, the number of adventitious roots was more noticeable at 0.25 mg L - I IAA plus 0.5 mg L -1 BAP. However, if just IAA was added led to higher number of regenerated roots compared to other treatments.Tétel Szabadon hozzáférhető Study of genetic transformation efficiency via organogenesis and embryogenesis in eggplant (Solanum melongena L. cv. Embti): effects of co-culture, temperature and kanamycin and hygromycin-based selection procedures(2002-10-16) Picoli, E. A. de T.; Brommonschenkel, S. H.; Cecon, P. R.; Da Silva, D. J. H.; Fári, M. G.; Otoni, W. C.The effects of kanamycin and hygromycin-based selection and co-culture temperature ranging from 22 to 28 °C upon eggplant transformation efficiency were evaluated. Both morphogenic pathways, somatic embryogenesis and organogenesis, were adopted using cotiledonary and hypocotyl explants, respectively. Somatic embryos were recovered in the presence of both antibiotics, although lesser escapes were observed in hygromycin-supplemented medium. Indeed, selection provided by this antibiotic was more efficient compared to kanamycin, nevertheless, shoot regeneration was not observed with hygromycin. Significant difference on the frequency of cotiledonary explants displaying callus (FEC) was observed as embryogenesis was concerned, although a higher number of embryos was observed in hygromycin selective media. The frequency of explants presenting callus (FEC), embryos (FEE) and shoots or buds (FERG) did not differ statistically for the tested co-culture temperatures, although higher regenerant number was observed at 24 °C.