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Tétel Korlátozottan hozzáférhető Tétel Szabadon hozzáférhető Examination of the efficacy of different fungicides against Macrophomina phaseolina and Sclerotinia sclerotiorum in laboratory conditions(2022-05-26) Csüllög, Kitti; Biró, Györgyi; Gonsalves, Joycline David; Sanga, Stoweka Maneno; Tuly, Nowrin Mostafa; Abushawish, Abdul Kareem; Tóth, Gyula; Vartek, Cintia; Ernhardt, Nóra; Tarcali, Gábor; Csótó, AndrásMacrophomina phaseolina and Sclerotinia sclerotiorum are two significant fungal pathogens of sunflower. M. phaseolina causes charcoal rot and ashy stem blight in several important crop species. Sclerotinia sclerotiorum causes white mold disease which can occur as middle stalk rot, head rot and premature plant death. Due to the wide host range of the two pathogens and their survival structures, crop rotation cannot provide sufficient protection against them. In our experiment, we selected two fungicides, Mirage and Prosaro, which are widely used in practice, and we tested their efficacy against the two pathogens. The efficiency of these fungicides was tested at a concentration of 10; 20; 50; 100 and 500 ppm. The Prosaro totally inhibited the mycelial growth of both pathogens at a concentration of 50 ppm, 100 ppm and 500 ppm. The Mirage caused total mycelial growth inhibition in all treatments against both pathogens.Tétel Szabadon hozzáférhető Examination of the efficacy of different fungicides against Macrophomina phaseolinaand Sclerotinia sclerotiorum in laboratory conditions(2022) Csüllög, Kitti; Biró, Györgyi; Gonsalves, Joycline David; Sanga, Stoweka Maneno; Tuly, Nowrin Mostafa; Abushawish, Abdul Kareem; Tóth, Gyula; Vartek, Cintia; Ernhardt, Nóra; Tarcali, Gábor; Csótó, AndrásTétel Korlátozottan hozzáférhető Fundamentals of Agricultural Extension and Education(DETEP, 2021-12) Tuly, Nowrin MostafaTétel Korlátozottan hozzáférhető Isolation, purification, identification and DNA sequencing of macrophomina phaseolina (Tassi) goid in sunflower plant by PCR methodTuly, Nowrin Mostafa; Tarcali, Gábor; DE--Mezőgazdaság- Élelmiszertudományi és Környezetgazdálkodási KarSunflower output globally is threatened by Macrophomina phaseolina (Tassi) Goid's charcoal rot. Macrophomina phaseolina can infect several wild and cultivated host species via seed, soil, or stubble. Controlling charcoal rot is challenging since disease infestation depends on several factors. There are few field infestation management options. Biological, cultural, and chemical controls have been used to manage this disease, but an effective strategy is unclear. The PCR was tried and modified at the University of Debrecen, Institute of Plant Protection according to their laboratory needs. This technique used ITS1 and ITS4 primers. A successful PCR method was developed after multiple repetitions and process reviews. The pure DNA was sequenced with the collaboration by Microsynth, Austria. The DNA sequence was then blasted on the NCBI website, yielding a 96.15% match. Macrophomina phaseolina can be detected early with this approach. Accurate diagnosis and early pathogen detection are crucial to plant disease management. It may be promising for managing and detecting this damaging disease. This work may indirectly reduce economic harm from the Macrophomina Phaseolina fungus, enabling sunflower and other crop cultivation in the future.