Szerző szerinti böngészés "Uray, Karen Lee"
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Tétel Korlátozottan hozzáférhető Pharmacogenomic investigation of SETMAR fusion gene on near-Haploid Lymphoblastoid Leukemia (HAP1) cell lineEsmandar, Zeina; Székvölgyi, Lóránt; Gyógyszerésztudományi Kar; DE--Általános Orvostudományi Kar; Uray, Karen Lee; Általános Orvostudományi Kar::Orvosi Vegytani IntézetGiven the lack of studied knowledge on the effects of SETMAR expression on drug sensitivity, we aimed to investigate various FDA-approved drugs impacting the viability of various genetically modified near-haploid human cell lines (HAP1) by applying high-content screening on the FDA-approved drug libraries in order to choose hits of active compounds for further analysis. HAP1 cells were treated with the hits (drugs) to evaluate how these drugs might influence cellular processes if we manipulated SETMAR expression levels in these cell lines.Tétel Korlátozottan hozzáférhető Phsiological rules of the multidrug resistance transporter P-glcoproteinFaraj, Nabeel; Bacsó, Zsolt; Department of Biophysics and Cell Biology; DE--Általános Orvostudományi Kar; Uray, Karen Lee; Általános Orvostudományi Kar :: Orvosi Vegytani IntézetThe thesis introduction talk about ABC transporters and their structures, P-glycoprotein and its mechanism of action, Cancer multidrug resistance, substrates, and inhibitors, The physiological role of Pgp, its expression and function in the immune cell populations, and its impact on the immune cells in cancer.In this work, our primary focus was on the evaluation of the Physiological expression of Pgp in human T lymphocytes, particularly in the primary human cytotoxic T lymphocytes (CTLs), besides its expression in two other lymphoid origin human tumor cell lines derived from hematological malignancies of the CD4+ Jurkat and the CD8+ TALL-1.Cells used were Primary human cytotoxic T lymphocytes, Jurkat, TALL-1, NIH 3T3 cells and NIH 3T3 MDR1 cells. Fluorescence microscopy and Flow cytometry were used to assay the calcein accumolation inside these cells with and without cyclosporin A.Tétel Korlátozottan hozzáférhető The effects of Danirixin, as a CXCR2 antagonist, on smooth muscle functionMesrati, Farah; Uray, Karen Lee; Általános Orvostudományi Kar::Orvosi Vegytani Intézet; DE--Általános Orvostudományi Kar; Szöllősi , Attila Gábor; Általános Orvostudományi Kar::Immunológiai IntézetThis research project was conducted in the frame of identifying the possibility of Danirixin use as a future treatment for ileus by proving the direct effect of danirixin on smooth muscle cell function through its signalling as a CXCR2 antagonist and investigating the effects of danirixin treatment on the overall proliferation and differentiation of the human smooth muscle cells.Tétel Korlátozottan hozzáférhető The role of HCN2 in macrophage cytokine releaseFrangu, Rrezon; Uray, Karen Lee; Általános Orvostudományi Kar::Orvosi Vegytani Intézet; DE--Általános Orvostudományi Kar; Koncz, Gábor; Általános Orvostudományi Kar::Immunológiai IntézetThis study explores the role of hyperpolarization-activated cyclic nucleotide-gated HCN channels in macrophage inflammation and their potential impact on intestinal motility. While HCN2 inhibition is known to decrease intestinal motility, its involvement in the development of ileus remains unclear. The research focused on HCN channels, particularly HCN2, which are crucial for cellular excitability and pacemaking, and are now recognized for their role in immune modulation. THP1 cells were treated with the HCN channel blocker ZD7288 or a vehicle control (DMSO) and subjected to cyclic stretch, with cytokine release measured to assess inflammation. Using the FlexCell system to apply mechanical stretch to activated THP1 cells, results showed a significant reduction in macrophage cytokine release following HCN2 inhibition. These findings enhance our understanding of HCN2 channels in macrophage inflammation and suggest their potential as therapeutic targets.