Szerző szerinti böngészés "Zacharia, Ezra"

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    Studying the Interaction Between Hyperactive PI3K-AKT and NF-κB Pathways in Differentiated Acute Promyelocytic Leukaemia (APL) Cell Lines
    Zacharia, Ezra; Jambrovics, Károly; Debreceni Egyetem::Általános Orvostudományi Kar::Biokémiai és Molekuláris Biológiai Intézet; DE--Általános Orvostudományi Kar; Demény, Máté Ágoston; Türk-Mázló, Anett; Debreceni Egyetem::Általános Orvostudományi Kar::Orvosi Vegytani Intézet; Debreceni Egyetem::Általános Orvostudományi Kar::Immunológiai Intézet
    Acute Promyelocytic Leukaemia (APL) is induced by the blocking of normal myeloid differentiation by the PML-RARA fusion oncoprotein, produced by the balanced translocation t(15;17) (q22;q12-21). All Trans Retinoic Acid (ATRA) treatment can be used as differentiation therapy in patients with APL, resulting in terminal differentiation of promyelocytes to neutrophil granulocytes. This process can be replicated in-vitro by the administration of ATRA to NB4 cells. Also identified as effective treatment for APL is the drug arsenic trioxide (ATO), which displays synergism when combined with ATRA, prolonging further survival of APL patients in a dose-dependent manner. ATO treatment can lead to complete remission in relapsed APL patients, inducing partial differentiation and apoptosis in those cells. This effect may be due to endogenous superoxide production following arsenic exposure by ATO. In the formation of functioning neutrophil granulocytes, thousands of gene up- and down-regulation processes are involved. A complication of ATRA therapy is the so-called ‘differentiation syndrome’ which causes an acute exaggerated inflammatory response. This study considers inhibition of the PI3K-AKT cellular signalling pathway as a target to achieve better results in differentiation therapy. As such, PHT (combined with ATRA and ATO) in increasing concentrations over time resulted in reduced activity of inflammatory (NF-κB) and apoptotic (FOXO3A) pathways, with reduced production of pro-inflammatory cytokines.