Szerző szerinti böngészés "Zegeye, Zemenu Birhan"

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    Application of laparoscopic artificial insemination in a conventional lacaune sheep farm using frozen-thawed semen
    Zegeye, Zemenu Birhan; Vass, Nóra; DE--Mezőgazdaság- Élelmiszertudományi és Környezetgazdálkodási Kar
    Due to the complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, this has resulted to a challenge in regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate (PR) and lambing rates (LR) of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen-semen from France originating from two rams of the Lacaune breed. Ram semen provided was categorized A and B to distinguish the identity of the two rams, whereas the ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the LAI technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and lambing rates results were 31.57% (6/19) and 42.10% (8/19) respectively. Out of 2 ewes in the one-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age group, 1 ewe in two-year-old age group and 16 ewes in four-year-old age group). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of lambing rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B respectively. There was no significant difference (p>0.05) in pregnancy and lambing rates based on semen collected from the two rams. Finally, in this research study, ram semen had no significant effect on pregnancy and lambing rates using laparoscopic AI on the flock. This could be due to the fact that the rams had quality semen. Furthermore, it could be due to the fact that the rams were of the same breed hence there was no difference between ram semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.
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    TételSzabadon hozzáférhető
    Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen
    (2020-12-01) Zegeye, Zemenu Birhan; Vass, Nóra; Tomano, Andualem
    The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in undeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.
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    Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary
    (2024-06-03) Zegeye, Zemenu Birhan; Magyar, Károly; Novotni-Dankó, Gabriella
    The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.
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