Szerző szerinti böngészés "Zenke, Petra"

Megjelenítve 1 - 3 (Összesen 3)
  • Nincs kép
    TételSzabadon hozzáférhető
    Evaluation of Maternal Genetic Background of Two Hungarian Autochthonous Sheep Breeds Coming from Different Geographical Directions
    (2022) Gáspárdy, András; Zenke, Petra; Kovács, Endre; Annus, Kata; Posta, János; Sáfár, László; Maróti-Agóts, Ákos
  • Nincs kép
    TételKorlátozottan hozzáférhető
    Population genetic indices in Hungarian Cikta sheep
    Kovács, Endre; Tempfli, Károly; Shannon, Andrew; Zenke, Petra; Maróti-Agóts, Ákos; Sáfár, László; Bali Papp, Ágnes; Gáspárdy, András; egyetemi oktató, kutató
    Samples of 72 animals from three flocks were analysed using nine microsatellites in order to evaluate the connection between existing populations of Cikta sheep, an indigenous breed in Hungary. Cikta was a well-known and popular breed until 1960-70. Nearly 30,000 animals were recorded in 1947 whereas the breed came close to extinction in 1975 when only 112 animals remained and genetic preservation programme was launched. Today's population is considered stable with approximately 1000 record animals. Fluorescent-labelled oligonucleotides and fragment length analysis was applied for microsatellite genotyping. Overall, observed and effective allele numbers were 5.63±1.71 and 3.76±1.10, respectively. Mean Fis (-0.18±0.12) and Fit (-0.13±0.11) values indicated heterozygous excess. Considerably low mean Fst (0.04±0.03) and discriminant analysis revealed lasting effects of the 1970s bottleneck as three analysed populations showed slight genetic differentiation. Three of the nine microsatellites significantly (P<0.05) deviated from Hardy-Weinberg equilibrium, namely BM8125, CSSM47, and MAF214. Results can be applied in mating plans to maintain existing diversity in the breed.
  • Nincs kép
    TételKorlátozottan hozzáférhető
    STR diversity of a historical sheep breed bottlenecked, the cikta
    Kovács, Endre; Tempfli, Károly; Shannon, Adrian; Zenke, Petra; Maróti-Agóts, Ákos; Sáfár, László; Bali Papp, Ágnes; Gáspárdy, András; PhD hallgató; egyetemi oktató, kutató
    The population structure of the endangered Cikta sheep breed was evaluated by means of nine microsatellite polymorphisms. Seventy-two individuals from three flocks were sampled to determine genetic indices in the Hungarian population. Overall, average observed and effective allele numbers were 5.63 and 3.76, respectively. Discriminant analysis based on genotype frequencies revealed moderate genetic diversity among Cikta flocks, since only three loci (OarCP49, CSSM47 and OarHH41) contributed significantly (P<0.05) to differences between subpopulations. Low squared Mahalanobis distances from group centroids also confirmed that the breed is almost equally represented by the three flocks. Moderate level of diversity between flocks was attributed to the long-term effects of a population bottleneck dating back to the 1970s. Negative average F1s value (-0.18) indicated heterozygote excess. Chi-squared tests identified significant (P<0.05) deviation from HWE in the case of BM8125, CSSM47, and MAF214 markers. Continuous microsatellite information is required for the preservation of rare alleles and diversity in Cikta sheep.