Determination of fatty acid composition of oils by mass spectrometry
Kötet címe (évfolyam száma)
The aim of my thesis was the determination of fatty acid content in the oils using mass spectrometry. I investigated the distribution of the fatty acid composition in beard oil, coconut oil, fish oil, palm oil and sunflower oil using GC-MS. Saponification was used as the first step in sample preparation and then I prepared the free fatty acid. HCl and Methanol is added to the sample to produce the FAMEs (Fatty acid methyl esters) done by acid catalyzed process. After this I identified these fatty acids by comparing their retention times to the FAMEs standard. Also using the chromatogram obtained from this experiment I could compare the compounds with the similarity search in the mass spectra library. Based on this I identified the various fatty acid compounds and determine the name.