Transcriptomics analysis of IDO-1 overexpressing breast cancer

Abstract

This thesis investigates the impact of IDO1 overexpression on gene expression in triple-negative breast cancer cells using RNA sequencing and RT-qPCR validation. The study addresses an important aspect of cancer biology by exploring the role of IDO1 beyond its established function in immune suppression. The methodology is appropriate and combines high-throughput transcriptomic analysis with experimental validation of selected genes. The results demonstrate that IDO1 influences multiple cellular processes, including transcription, RNA processing, DNA repair, and metabolic regulation, highlighting its broad contribution to tumor progression. The findings provide valuable insights into the molecular mechanisms underlying breast cancer development and may support the identification of novel therapeutic targets. Overall, this work represents a well-designed and relevant contribution to understanding the role of IDO1 in triple-negative breast cancer.