Micropropagation of Rudbeckia hirta L. from seedling explants
| dc.contributor.author | Szarvas, Pál | |
| dc.contributor.author | Zsila-André, Anikó | |
| dc.contributor.author | Kovács, Zoltán | |
| dc.contributor.author | Fári, Miklós Gábor | |
| dc.date.accessioned | 2021-06-28T10:59:24Z | |
| dc.date.available | 2021-06-28T10:59:24Z | |
| dc.date.issued | 2006-05-23 | |
| dc.description.abstract | We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding. | en |
| dc.format | application/pdf | |
| dc.identifier.citation | Acta Agraria Debreceniensis, No. 22 (2006) , 53-59 | |
| dc.identifier.doi | https://doi.org/10.34101/actaagrar/22/3191 | |
| dc.identifier.issn | 2416-1640 | |
| dc.identifier.issue | 22 | |
| dc.identifier.jatitle | Acta agrar. Debr. | |
| dc.identifier.jtitle | Acta Agraria Debreceniensis | |
| dc.identifier.uri | https://hdl.handle.net/2437/316693 | en |
| dc.language | en | |
| dc.relation | https://ojs.lib.unideb.hu/actaagrar/article/view/3191 | |
| dc.rights.access | Open Access | |
| dc.subject | Rudbeckia hirta L. | en |
| dc.subject | in vitro | en |
| dc.subject | seedlings | en |
| dc.subject | micropropagation | en |
| dc.title | Micropropagation of Rudbeckia hirta L. from seedling explants | en |
| dc.type | folyóiratcikk | hu |
| dc.type | article | en |
Fájlok
Eredeti köteg (ORIGINAL bundle)
1 - 1 (Összesen 1)