The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon

A tétel rövid nézete
dc.creatorQiu, Z.
dc.creatorBársony, Cs.
dc.creatorBisztray, Gy.
dc.creatorVelich, I.
dc.date1999-09-13
dc.date.accessioned2021-06-28T09:57:08Z
dc.date.available2021-06-28T09:57:08Z
dc.descriptionSome parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.  
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/42
dc.identifier10.31421/IJHS/5/3-4/42
dc.identifier.urihttp://hdl.handle.net/2437/313972
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/42/42
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 5 No. 3-4 (1999); 46-49.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/5/3-4
dc.subjecttransformation
dc.subjectmuskmelon
dc.subjectaAgrobacterium-mediated
dc.subjectherbicide
dc.subjectresistance
dc.titleThe Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
Fájlok
Eredeti köteg (ORIGINAL bundle)
Megjelenítve 1 - 1 (Összesen 1)
Nem elérhető
Név:
pdf.pdf
Méret:
1.77 MB
Formátum:
Adobe Portable Document Format
Letöltés
Gyűjtemények
International Journal of Horticultural Science