The Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelon

A tétel rövid nézete
dc.contributor.authorQiu, Z.
dc.contributor.authorBársony, Cs.
dc.contributor.authorBisztray, Gy.
dc.contributor.authorVelich, I.
dc.date.accessioned2021-06-28T09:57:08Z
dc.date.available2021-06-28T09:57:08Z
dc.date.issued1999-09-13
dc.description.abstractSome parameters involved in Agrobacterium-mediated transformation in muskmelon Hales best (HBS) were studied. Cotyledon explants excised from 3.5-day-old seedlings were co-cultivated with Agrobacterium tumefaciens harbouring binary vectors which contained GUS and BAR genes. After co-cultivation on a low pH medium, explants were transferred to selective medium, with higher pH, containing Claforan and Finale. The medium was changed every two weeks till shoots were induced. All shoots rooted on MS medium supplemented with 0.3 mg/L IBA. These parameters combined as a whole led to successful transformation. The expression of the introduced gene construct was confirmed by GUS staining of shoot segments.  en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 5 No. 3-4 (1999) , 46-49.
dc.identifier.doihttps://doi.org/10.31421/IJHS/5/3-4/42
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue3-4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/313972en
dc.identifier.volume5
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/42
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjecttransformationen
dc.subjectmuskmelonen
dc.subjectaAgrobacterium-mediateden
dc.subjectherbicideen
dc.subjectresistanceen
dc.titleThe Effects of Some Parameters on Agrobacterium-Mediated Transformation in Muskmelonen
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)