International Journal of Horticultural Science (DE-journals)

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Megjelenítve 1 - 20 (Összesen 1016)

Szabadon hozzáférhető
Impact of different growing media on propagation of bougainvillea (Bougainvillea spp.) through hardwood stem cuttings in Chitwan, Nepal
(2025-07-08) Panthi, Swikriti; Pant, Mukesh; Chand, Roshan; Devkota, Toran
An experiment was conducted in Rampur, Chitwan, Nepal, in a Randomized Complete Block Design (RCBD) with five treatments: T1 (sand:soil, 1:1) [control], T2 (sand:cocopeat:perlite, 1:1:1), T3 (perlite:sand, 1:1), T4 (sand:cocopeat, 1:1), and T5 (peat moss:perlite, 1:1). Each treatment was replicated four times with a total of 300 planted cuttings. The key parameters evaluated were sprouting percentage, shoot growth (length, leaf number, and branch number), and root development (length, number, fresh, and dry biomass). Analysis of data was done using R-Studio, and Duncan’s Multiple Range Test (DMRT) was performed at a 5% significance level. The results revealed that peat moss:perlite (1:1) demonstrated the highest shoot growth with shoot length of 6.00, 14.38 and 28.78 cm at 30, 60, and 90 Days After Planting (DAP) respectively. Leaf number were 6.25, 13.12, and 19.57, while branch numbers were 1.76, 2.00, and 2.18 at the same intervals., Additionally, the fresh root weight was recorded 8.74 g. Sand:cocopeat:perlite (1:1:1) recorded the highest sprouting percentage (81.66%) and root length (17.11 cm), while sand:cocopeat (1:1) achieved the highest root number (18.37) and dry root weight (0.99 g). Perlite:sand (1:1) exhibited moderate performance with sprouting percentage 69.99%, and root length 12.56 cm. In contrast, control (sand:soil) showed the least favorable results with the lowest sprouting percentage (58.33%), the fewest number of roots (7.81), and the minimum root length (9.73). This study concludes that peat moss:perlite (1:1) is the most effective growing medium for bougainvillea propagation, offering a practical solution to enhance rooting success and growth performance. Sand:cocopeat (1:1) with maximum dry weight of root and maximum number of roots emerged as a reliable alternative, proving the effectiveness of the medium.
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Left, right, up and downstage: leaves and lateral roots histological trait prospection for drought tolerance in commercial Coffea arabica cultivars
(2025-07-08) Godinho, Natália; de Souza, Tereza; Rocha, Maria Eduarda; Pérez-Molina, Junior Pastor; Picoli, Edgard; Jacomini, Franciely; Ladeira, Josimar; Vilela, Diego; Aguiar, Vinicius; Mendonça, Abelardo; Ribeiro, Marcelo; Pereira, Antônio; de Oliveira, Antônio Carlos; de Carvalho, Maria Antônia; Pedrosa, Adriene; Ferreira, Williams
The climate change and water deficit challenges plant producers all over the world, and have consequences to coffee production and quality. In this research we have approached anatomical traits from vegetative organs of 13 Coffea arabica genotypes, selected based on their contrasting behavior to water deficit. Leaf blade, petiole and primary root cross sections were evaluated, and the epidermal, fundamental, and vascular tissues descriptive anatomy, histometric and histochemistry examined. Despite all plants were in the same environment (CEPC/EPAMIG, Patrocínio, MG, Brazil), there were differences among the genotypes and groups of more tolerant and more susceptible accesses. Petiole cross section, vascular tissue and phloem and cambium; and percentage of stele, pericycle and phloem and cambium in primary roots exhibited differences among the contrasting genotypes, highlighting an inborn association of vascular tissue and other features with water deficit resistance. This association was observed in the mild to medium correlations among vascular tissue, epidermis, phloem and cambium in roots and petioles. Possible relation of qualitative traits such as the lignification of root epidermis, lipidic substances in outer cortical cell layers, and area/number of cell layers in the cortex are approached as possible traits in the seek for water deficit tolerance in C. arabica .
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Linking bark anatomy to Eucalyptus Physiological Disorder (EPD) in commercial clones
(2025-07-08) Picoli, Edgard; Jacomini, Franciely; Ladeira, Josimar; Almeida, Maria Naruna; Vidaurre, Graziela; Moulin, Jordão; Zauza, Edival; Guimarães, Lúcio; Isaias, Rosy Mary; Balmant, Kelly; da Costa, Weverton
Abiotic stresses trigger the Eucalyptus Physiological Disorder (EPD) which poses a threat to planted and native stands. This research seeks links between eucalyptus bark histological features and EPD, in which the descriptive bark anatomy and histochemistry are approached. Barks from 5-year eucalyptus trees, from commercial clones of E. grandis , E. urophylla and its hybrids, were collected at breast height (DBH), and 50% and 75% of the commercial height, and evaluated. The eucalyptus bark consisted of a periderm (or rhytidome) and a secondary phloem with conspicuous solitary sieve tube elements (STE). The outer bark revealed a secondary phloem with collapsed STE, whereas its inner counterpart displayed non-collapsed STEs. A region crowded with calcium oxalate (CaOx) crystals in axial parenchyma, covering the non-collapsed and partially overlapped collapsed secondary phloem, was observed. Eucalyptus barks exhibited similar anatomical organization at DBH, 50% and 75% of the commercial height, irrespective of expected EPD phenotype or scores. Notwithstanding, there are qualitative differences that are associated with the proportion of non-collapsed phloem and phloem with crystals, which were higher in the tolerant clones and in trees with score 0. The more resistant clones or samples with lower EPD scores exhibited a higher proportion of the regions of living phloem, phloem with CaOx crystals, and non-collapsed phloem. These results support the hypothesis that an increased proportion of STE collapse will occur concurrently with elevated EPD scores and are the basis for an ongoing histometric approach.
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Optimizing disinfection protocols for yam explant regeneration in plant tissue culture
(2025-07-08) Komolafe, Omotayo; Oyinloye, Oyindamola H.; Ogbimi, Ejeoghene R.; Hassan, Kasali. O.; Pelemo, Olugboyega S.
Yam ( Dioscorea species ), does not produce commercially viable seeds, and asexual propagation is faced with challenges resulting from carried-over infections from previous generations. Contamination is a prevalent problem in Plant Tissue Culture (PTC), making the development of cost-effective and efficient disinfection protocols crucial for successful PTC. This study aimed to evaluate the effectiveness of yam explant disinfection protocols using various immersion timings, disinfectant concentrations (including ethanol (C 2 H 5 OH), sodium hypochlorite (NaOCl), and hydrogen peroxide (H 2 O 2 )), and in single or combined disinfectants application. Twenty treatment combinations and one control were assessed on yam vines for Disinfection Efficiency (DE%), Negative Disinfection Effect (NDE%), and the regeneration of shoots and roots (SN & RN) from the culture after 21 days. The study showed that varying immersion times did not significantly impact the evaluated parameters. However, different concentrations of disinfectants resulted in diverse NDE responses. Surprisingly, higher concentrations of NaOCl led to reduced NDE, whereas lower concentrations increased NDE. On the contrary, higher concentrations of H 2 O 2 increased NDE, while lower concentrations decreased it. Shoot and root regeneration rates were also significantly impacted by the choice of disinfection protocol. The research concluded that dual disinfection protocol, specifically 70% ethanol for 7 minutes followed by NaOCl, was most effective for eliminating surface-borne contaminants and achieving successful in vitro propagation of yam plantlets. This method offers a cost-effective solution for establishing microbe-free tissue culture yam plantlets and provides a basis for future research on other Dioscorea plants.
Szabadon hozzáférhető
Differing effects of grass and polythene mulches on sweet potato productivity in derived savannah agro-ecology
(2025-07-08) Amuji, Felix; Ezema, Perpetual; Osadebe, Vivian; Obi, Justina; Obalum, Sunday
Sweet potato as a food crop that is grown for both its tuberous roots and vegetative parts, the latter of which also serves as animal feed. Its production in the savanna regions can be limited by high evaporative demand, especially where soils with low water retentivity are involved. This work studied the effects of various locally available polythene as mulch materials for sweet potato growth in derived savanna agro-ecology. An experiment was thus conducted at Nsukka, a derived savannah in southeastern Nigeria, with orange fleshed variety of sweet potato. Treatments were polythene mulches of contrasting colours (black, white, red and blue polyethylene), including also organic mulch (dry grasses) as reference and a no-mulch control. The experiment was executed in two successive cropping seasons, and the data averaged and analysed. Agronomic and weed incidence data collection was done fortnightly from 2 nd to 10 th week of planting. The results showed that the polythene mulches (especially the black one) were better in the control of weeds. Furthermore, the sweet potato yields were affected significantly (p < 0.05) by mulching on the total number of tubers for each plant with grass-mulched plots showing the highest value (36) and transparent polyethylene-mulched plots the lowest (19). Also, total weight of tubers per plant at harvest differed ( p < 0.05), with grass-mulched plots showing the highest value (2.39 kg), followed by the control (no mulching) plots (1.62 kg), then the coloured polyethylene mulched plots with the red polyethylene-mulched plots as the lowest (0.64 kg). This study recommends the use of organic mulch or no mulching in sweet potato production in this agro-ecological zone as they produced the best yields.
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Effect of different biochar on acidic soil, growth, and nutritional status of oil palm (Elaeis guineensis Jacq.) under the nursery conditionrsery condition.
(2025-07-08) ALEIADEH, HEMAM
Biochar is a widely known organic amendment that enhances plant growth and productivity through improving soil condition and fertility. However, plant and soil response differently to various types of biochar. Therefore, the major objective of the present study was to determine the effect of two types of biochar; vetiver grass biochar (VGB) and pineapple leaf biochar (PLB) on selected soil properties and understand their role in improving plant growth and nutritional status. Net house experimental study with oil palm ( Elaeis guineensis Jacq.) seedlings was conducted at the farm unit of UiTM Samarahan campus for 240 days between November 2022 and July 2023 in order to understand the impact of different application rates (25, 50, 75, and 100%) of vetiver grass biochar and pineapple leaf biochar on selected soil properties including pH, electrical conductivity (EC), nitrogen, phosphorus, and potassium content, plant growth parameters including plant height, bole diameter, leaf number, SPAD chlorophyll content, plant dry weight, and plant’s NPK content. The experimental design used is randomized complete block design (RCBD) with 8 treatments, 3 replicates, and each replicate has 6 plants. The analysis of variance (ANOVA) and DMRT were used to analyze the collected data from soil and plant parameters. The analysis process was accomplished using SAS package (P<0.05): version 9.4 (SAS, 2013). Based on the results, vetiver grass biochar and pineapple leaf biochar enhanced soil pH, EC, and NPK content. They also improved the growth and nutritional status of oil palm seedlings. Soil enhancement was the best with 100% pineapple leaf biochar. In addition, the highest mean value of plant height, bole diameter, leaf number, SPAD chlorophyll content, plant dry weight, and plant’s NPK content was observed in plants treated with 100% pineapple leaf biochar. Therefore, vetiver grass biochar and pineapple leaf biochar have the potential to enhance acidic and poor-fertile soil, and improve oil palm seedlings growth and nutritional status.
Szabadon hozzáférhető
Efficacy of selected botanical extracts against Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) on tomato
(2025-07-08) Nabutse, Daniel Kepha; Saidi, Mwanarusi; Ogweno, Joshua
Tuta absoluta Meyrick, also known as the tomato leaf miner, is a destructive pest that feeds on tomato plants, causing significant economic losses to tomato farmers globally. Traditional methods of controlling Tuta absoluta involve the use of synthetic pesticides, but these methods have drawbacks such as environmental pollution, development of resistance by the pest, and negative effects on non-target organisms. As a result, there is a growing interest in the use of botanical extracts as alternative and safer pest control options. Three bioassay experiments were conducted to test the insecticidal, antifeedant and repellency effects of botanical extracts on Tuta absoluta . The experiments were conducted as completely randomized design (CRD) with three (3) replicates. There were eleven treatments; pyrethrum, neem, warbugia, each at different concentration levels of (8%, 10%, 12%) and controls positive (Indoxacarb 150g/L) and negative (distilled water). In all the bioassay conducted, the effects of all treatments increased over time, except for distilled water control. Synthetic chemical Indoxacarb exhibited significantly stronger insecticidal properties compared to neem, warburgia and pyrethrum extracts. Application of neem at 8%, 10% and 12% and pyrethrum at 12% had higher antifeeding effect on Tuta absoluta compared to the other treatments while warburgia at 8% concentration and indoxacarb had the lowest repellency effect compared to the other plant extracts.
Szabadon hozzáférhető
Yield and fruit quality response to foliar application of biostimulants in an apple orchard
(2025-07-08) Csihon, Adam; Sipos, Marianna; Holb, Imre
Nutrient supply plays an important role in fruit production technology. Among the methods of nutrient management, the application of foliar fertilization has become an essential tool to harmonize the vegetative and generative performance of fruit trees. This study was conducted to determine the effect of four foliar fertilizers (Bistep, brown juice, Trichoderma harzianum , Clonostachys rosea ) on yield and fruit quality in an apple orchard with the cultivar ‘Pinova’, at the University of Debrecen in Hungary. Trees were trained to a slender spindle canopy with a spacing of 4 × 1 m. Treatments were applied five times during different phenological phases in 2021 and 2022. Based on our results, biostimulants had a positive effect on fruit yield and fruit quality, but extreme weather events also influenced the data. In 2021, the effect of the treatments on yield data could not be observed (10.1–13.5 kg/tree; 0.16–0.24 kg/cm²). However, in 2022, plants sprayed with Bistep, T. harzianum , and C. rosea reached 28.7–31.2 kg/tree (0.33–0.46 kg/cm²), while control trees produced only 19.8 kg/tree (0.20 kg/cm²). Fruit size development improved with an increase of 2–6 mm over the two years. Among the treatments, Bistep was able to enhance fruit surface coloration to a greater extent in 2021, as red skin color reached 44% for this foliar fertilizer, while control trees presented only 27%.
Szabadon hozzáférhető
Effects of wastewater irrigation on soil physico-chemical properties and vegetables quality: A review
(2025-07-08) Sibanda, Justice; Parwada, Cosmas; Mvumi, Cluver
This study analyses the use of raw urban wastewater for irrigation and its effects on soil characteristics and heavy metal pollution within crops. There is rampant use of urban waste water by small scale urban vegetable farmers in Zimbabwe. This is posing health hazards to the consumers of urban vegetables and decrease in soil functioning. The significance of this study was to appraise the research study related to the use of urban waste water to answer the research question: What is the impact of waste water on selected soil physico-chemical properties and quantities of heavy metals in vegetables irrigated with waste water? A systematic review of 3100 articles from PubMed, Scopus, Web of Science, and Google Scholar (2010–2024) was performed within PRISMA guidelines. After excluding irrelevant studies, 49 from Nigeria, Bangladesh, Iran, Egypt, India, Pakistan, China, Saudi Arabia, and DRC were selected for analysis. These countries were found to have a lot of literature on urban waste water irrigation. This literature review identified an overview of existing literature on urban waste water irrigation and synthesise findings to provide a comprehensive overview of the topic The results showed (P<0.05) soil properties changed significantly: pH (6.0–7.0 to 4.5–5.5), electrical conductivity (0.2–0.4 to 1.5–2.0 dS/m), organic matter (2–3% to 4–6%), cation exchange capacity (10–15 to 20–25 meq/100g) all aligning with the intended outcomes. The level of nutrients (N, P, K) shifted alongside the source of wastewater. Soil also accumulated heavy metals (Zn, Cd, Pb, Fe, Mn, Cu) while the crops accumulated unsafe levels of Pb (2.5 mg/kg), Cd (1.2 mg/kg), and Cr (3.1 mg/kg) which surpassed WHO/FAO limits. The results highlighted the concern for health and environmental hazards. Treatment of the wastewater, monitoring of the soil, and stricter guidelines are needed for safe agricultural practice.
Szabadon hozzáférhető
Effect of nitrogen on growth and yield of chili (Capsicum annuum var. ‘Marshal’) in Chitwan, Nepal
(2025-07-08) Thapa Tamang, Akash; Bhattarai, Ranjan; Pant, Mukesh; Rimal, Basanta Kumar; Rokaya, Pradeep Raj
A field experiment was conducted from April to July 2024 in Rampur, Chitwan, to evaluate the performance of different levels of nitrogen on the growth and yield attributes of chili. The Marshal variety of chili was tested under five different nitrogen doses (0, 30, 60, 90, and 120 kg/ha). The treatments were arranged in a one-factorial Randomized Complete Block Design (RCBD) with four replications . Observations were for different growth and yield parameters, and data were analyzed using R-studio. The results revealed that all growth and yield characters of chili were significantly influenced by nitrogen levels. The plants treated with 120 kg/ha nitrogen produced the maximum height (45.6 cm), canopy (604.5 cm 2 ), primary branch (8.67), leaf number (9.78), fruit length (6.26 cm), and fruit weight per plot (111.04 g). The 90 kg/ha nitrogen ranked second, with plant height of 43.12 cm, canopy cover 570.70 cm 2 , primary branch 7.30, leaf number 9.23, fruit length 5.87 cm, and fruit weight per plot 103.63 g. All parameters were significantly reduced in the control, where no nitrogen was applied. The highest yield was obtained in T 5 (3.07 t/ha) followed by T 4 (2.98 t/ha), T 3 (2.67 t/ha), and T 2 (2.32 t/ha). The findings of this study demonstrated that 120 kg/ha nitrogen level resulted in the highest values for all measured traits and was significantly greater than that of all other nitrogen levels. Therefore, it is concluded that nitrogen dose of 120 kg/ha is the most economical for achieving better growth and fruit yield in chili (variety ‘Marshal’).
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Agromorphological and nutritional quality profiles of fluted pumpkin (Telfairia occidentalis Hook F.) as influenced by cultivar, growing medium and soil amendment source
(2023-07-20) Oyetunde, O.; Benson, G. A. S.; Oyetunde, A. K.; Olalekan, O. J.; Chukwu, C. U.; Anari, K. O.
Fluted pumpkin ( Telfairia occidentalis Hook F.) is popular as food and feed around the world. Sixteen treatments were developed from factorial combinations of three factors: cultivar ( ugu elu and ugu ala ), growing medium (garden soil (GS) and white sand (WS)), and soil amendment source (poultry manure, NPK, supergro and no amendment). A pot experiment was conducted to investigate the agromorphological and nutritional traits of fluted pumpkin obtained from the treatments. Fresh leaves were analyzed for crude protein, crude fibre, crude lipid, total ash, phytate and nitrate concentrations. Data were subjected to analysis of variance and principal component analysis. Mean plots were used to explain the effects of the three factors and profiling was done using the GYT biplot. There were significant (p≤0.05/0.01) mean squares for measured traits, suggesting the possibility of selection among the treatments. Plants in GS consistently out-performed those in WS for shoot weight, leaf length, and number of leaves per plant possibly due to greater availability of nutrients in the GS. Inconsistent patterns observed in the proximate concentrations of pumpkin from the 16 treatments showed the role of interaction among the three factors. Principal component analysis identified some traits as contributors to differences among the treatments which can be basis of selection. Treatments 2, 4, 6, 8, 10, 11, 12, 13, 14, 15, and 16 might be useful to improve vegetative yield while 1, 3, 5, 7, and 9 could improve nutritional values of the fluted pumpkin.
Szabadon hozzáférhető
Effect of hydroponic and peat-free media in transplant production of Rudbeckia hirta varieties under different photoperiodic lighting and their photosynthetic parameters
(2021-08-10) Antal, G.; Kurucz, E.; Koncz, K.; Kaszás, L.; Fári, M. G.
The purpose of this research was to determine the effects of varieties, different light conditions (short day, long day, natural short day with light pollution), and different growing media (perlite, peat-free, peat-based, aeroponics system) on Rudbeckia hirta plant production under controlled conditions (greenhouse). The morphological effects of each treatment (photoperiodic lightings and media) on different Rudbeckia varieties determined at 11 weeks-old ’Napfény’, ’Toto Gold’, ’Autumn Colors’, ’Prairie Sun’ and 16 weeks-old ’Napfény’. Plantlets received 12 hours daylight did not initiate flowers, remained stage of the leaf rosette in case of all varieties. The 14 hours light treatment in the aeroponics system and the same treatment in perlite and control (natural short day with 14 hours light pollution) plantlets had developed inflorescences or flower buds. The inflorescence axis of ‘Napfény’ was appeared at 13 weeks under long-day conditions, with 1.7 (perlite) - 2.7 (aeroponics) flower buds in 16 weeks. ’Toto Gold’, ’Autumn Colors’, ’Prairie Sun’ varieties developed inflorescences at 8 weeks, 14 hours aeroponics system resulted in the most of flower buds (’Toto Gold’: 6.5, ’Autumn Colors’: 3.25,’Prairie Sun’: 4.8 flower buds) at 11 weeks. Long daylight manipulation could be minimized crop times and achieved flowering potted plants at 11 weeks. The peat-based and peat-free media effect was observed on ‘Autumn Colors’. The number of leaves of peat-free ‘Autumn Colors’ transplants (16.8-20.3) was significantly higher than peat-based media (13.5-15.5). Other morphological parameters were not affected by the media treatments.
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Vegetative and micropropagation potential of Piper guineense (Schumach and Thonn)
(2023-07-20) SAKPERE, A. M.; Ezenu, V. N.
The continuous loss of forest plants due to deforestation, and the increasing demand for Piper guineense because of its medicinal and food value, has put a permanent pressure on its population in the wild where it is collected. A method for conservation and mass propagation is therefore required. This research was undertaken to determine the optimal concentration of auxin needed for vegetative propagation and to investigate the potential of Piper guineense for micropropagation. The auxin optimization study of vegetative propagation was based on the use of two-nodal stem cuttings treated with five different concentrations of indole-butyric acid (IBA). Growth parameters such as the number of sprouted, rooted and survived cuttings among others were determined. To investigate the potential of Piper guineense for micropropagation, nodal explants were subjected to different sterilizing treatments using ethanol, NaOCl, mancozeb, streptomycin and Plant Preservative Mixture (PPM). The effect of plant growth regulators (PGRs) was tested on sterilized nodal explants using full strength Murashige and Skoog (MS) hormone-free media alone as control and MS media supplemented with PGRs (BA, NAA and KIN) at different concentrations and combinations. Significant differences were observed across the treatments for all growth parameters measured. However, 2000 ppm IBA significantly (p<0.05) influenced sprouting and rooting of the stem cuttings. Piper guineense explants have deep tissue contaminants, which cannot be eradicated by surface sterilization alone except double sterilization using PPM. On control media, neither shoot nor root response was observed while the highest percentage of induced roots was obtained from explants cultured on MS +1 mg/L BA + 0.25 mg/L NAA. Shoot induction was only achieved when BA was used alone and when subcultured on media supplemented with NAA, which generated roots.
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Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia
(2023-07-20) Biratu, W.; Molla , B.; Abebe , H.; Gebremeskel , H.
The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha -1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.
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Effects of salinity stress induced by hot spring water on tomato growth, yield and fruit quality under hydroponic cultivation in Japan
(2023-07-20) Musabyisoni, A.; Mutimawurugo, M. C.; Niyokuri, A. N.
The objective of this research was to test hot spring water as a source of salt to improve tomato quality under the hydroponic system. This research was conducted at Yamagata University, in Japan from February to July 2017. Salt stress was induced using salts of hot spring wastewater collected from Yupoka Onsen (Tsuruoka, Japan). The treatments were EC 2, 4, 8 12, and 16 mS/cm which were arranged in a Randomized Complete Block Design (RCBD) with five replications. Tomato plants were grown at EC 2 until flowering and then subjected to different EC concentrations until harvesting. The data were collected on plant growth parameters and fruit quality. Fruits were harvested at the red stage until the 5 th truss. The results showed that fruits' Soluble Solids Content, organic acid, Nitrate contents and Sugar: Acid ratio increased significantly at EC 16 mS/cm and in the upper trusses compared to EC 2 and in the lower trusses. In contrast, fruit weight significantly decreased at EC 16 mS/cm and in upper trusses. Leaf thickness, size and SPAD, and specific leaf weight significantly declined at EC 16 mS/cm and upper leaves compared to EC 2 and in lower leaves. Plant height started to decline significantly after three weeks of treatment at EC 16 compared to EC 2.
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Effects of biochar and inorganic fertiliser on the growth and yield of beetroot (Beta vulgaris L.) in Kenya
(2023-07-20) Kwizera, E.; Opiyo, A. M.; Mungai, N. W.
Beetroot ( Beta vulgaris L.) is a root vegetable packed with many nutritional benefits such as minerals and vitamins. Despite its importance in Kenya, farmers get about 30-35 t/ha which is significantly lower than the potential yield (68 t/ha). This is mostly attributed to low soil fertility. This study aimed to determine the response of the beetroot growth and yield on biochar and NPK. A 3×4 factorial experiment was carried out at Egerton University farm over two seasons to test the effects of biochar and NPK (17-17-17), under supplemental irrigation. Biochar (0, 5, 10 t/ha) was combined with NPK (0, 200, 300, 400 kg/ha). The combination of Biochar and NPK increased significantly ( p ≤ 0.05) beetroot growth and yield in two seasons. Treatment B10N400 showed the tallest plants (79.2 cm) at 90 days in season two, while the control resulted in the shortest (27.6 cm). Treatment B10N200 showed the biggest (213.2 cm 2 ) leaves at 90 days. The treatment B5N300 recorded the highest marketable yield (84 t/ha) in season two and the lowest was B0N0 with 2.6 t/ha. Sole application of NPK rates (200, 300, 400 kg/ha) increased significantly the growth and yield of beetroot compared to the control in both seasons. In season one, N300 (300 kg/ha) had 61.9 t/ha of the total yield, the control had the lowest. In season two, 300 kg/ha had 83 t/ha of total yield. Biochar increased beetroot growth and yield in season 2. Treatment B5 recorded the highest marketable yield of 61.2 t/ha, while the control showed the lowest of 53 t/ha.
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Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
(2023-07-20) Esfahani, R. N.; Khaghani, S.; Mortazaeinezhad, F.; Azizi, A.; Gomarian, M.
The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.
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Participatory evaluation of tomato (Lycopersicon esculentum Mill.) varieties under irrigation conditions at Abergelle district, Eastern Amhara
(2023-07-20) Mihiretu, A.; Asresu, M.
Participatory on-farm evaluation of improved tomato varieties (‘Melka salsa’, 'Roma VF' and 'Kochero’) against the local tomato variety was carried out during the 2019/2020 growing season involving six farmers of Abergelle district, Eastern Amhara. The objective of the experiment was to assess the performance of different tomato varieties for the farmers, then to collect their feedback. Based on the actual and farmers’ preference data, the analysis underscores the better performance of improved tomato varieties over local varieties by most yield-related attributes. Average marketable fruit yields of ‘Melka salsa’, ‘Roma VF’, ‘Kochero’, and the local variety were 4.62, 3.88, 3.64, and 3.10 ton ha -1 , respectively. The improved varieties thus had a yield advantage of 72.38%, 37.14%, and 25.72% over the local variety in that order. Among improved tomato varieties, ‘Melka salsa' provided the highest fruit yield on top of owing the highest score of overall preference attributes rank. Scale-wide diffusion of ‘Melka salsa’ tomato variety is therefore suggested for similar and potential agro-ecologies. Biological scientists also should take farmers’ preference attributes and feedback as a backup for future breeding and adaptation studies on tomato varieties.
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Agrobacterium transformation of Rhodiola sp.: current status and limitations
(2024-07-16) Javid, A.; György, Zs.
The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19 th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.
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Calla lily soft rot causal agents, symptoms, virulence and management: a review
(2023-07-20) Niyokuri, A. N.; Nyalala, S.
Bacterial soft rot is a polyetiological disease attacking calla lily [ Zantedeschia spp (L.) Spreng.]. This disease has reduced the commercial value of this crop. This work aims to review scientific information to give an insight into calla lily soft rot causal agents, symptoms, factors favouring the disease, virulence mechanisms and management strategies. Special emphasis is put on the current progress with regards to understanding calla lily mechanisms of resistance to soft rot and their potential for the development of tolerant/resistant cultivars with commercial traits.

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