Study of different factors of grapevine regeneration systems and genetic transformation

A tétel rövid nézete
dc.contributor.authorNovák, E.
dc.contributor.authorZok, A.
dc.contributor.authorOláh, R.
dc.date.accessioned2021-06-28T10:02:59Z
dc.date.available2021-06-28T10:02:59Z
dc.date.issued2008-12-08
dc.description.abstractThe most limitating factor for successful transformation is the absence of high-yielding regeneration protocols. However, the anther-derived embryogenic culture is an optimal technique for genetic transformation and it has been widely applied in many important cultivars, but the necessity of further development of regeneration systems has been proved. We attempted to produce somatic embryos on a wide range of genotypes from various tissues; leaves, petioles, stem segments. We started the examination of grapevine regeneration via organogenesis, succeeded in inducing shoot from the meristematic tissue of the base of bud by testing induction medium contained different concentrations of two types of hormones. To optimize the conditions of the Agrobacterium-mediated transformation, we studied the effectiveness of different Agrobacterium-treatments, the use of antioxidants and the sufficient quantity of kanamycin for selection of transformed cells.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 14 No. 4 (2008) , 33-36.
dc.identifier.doihttps://doi.org/10.31421/IJHS/14/4/1529
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314847en
dc.identifier.volume14
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/1529
dc.rights.accessOpen Access
dc.subjectAgrobacterium tumefaciensen
dc.subjectorganogenesisen
dc.subjectsomatic embryogenesisen
dc.subjecttransgenic planten
dc.titleStudy of different factors of grapevine regeneration systems and genetic transformationen
dc.typefolyóiratcikkhu
dc.typearticleen
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