Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA

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dc.creatorSzőke, A.
dc.creatorKiss, E.
dc.creatorToldi, O.
dc.creatorHeszky, L.
dc.date2006-09-26
dc.date.accessioned2021-06-28T09:59:59Z
dc.date.available2021-06-28T09:59:59Z
dc.descriptionTransgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.
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dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/683
dc.identifier10.31421/IJHS/12/4/683
dc.identifier.urihttp://hdl.handle.net/2437/314420
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/683/682
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 12 No. 4 (2006); 75-79.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/12/4
dc.subject-phosphofructo-2-kinase/fructose 2,6-bisphosphatase
dc.subjectAgrobacterium tumefaciens
dc.subjectfructose 2,6-bisphosphate
dc.subjectRT-PCR
dc.subjecttransgenic carnation
dc.subjectDianthus. chinensis
dc.subjectDianthus caryophyllus
dc.titleProduction of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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International Journal of Horticultural Science