Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA

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dc.contributor.authorSzőke, A.
dc.contributor.authorKiss, E.
dc.contributor.authorToldi, O.
dc.contributor.authorHeszky, L.
dc.date.accessioned2021-06-28T09:59:59Z
dc.date.available2021-06-28T09:59:59Z
dc.date.issued2006-09-26
dc.description.abstractTransgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 12 No. 4 (2006) , 75-79.
dc.identifier.doihttps://doi.org/10.31421/IJHS/12/4/683
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314420en
dc.identifier.volume12
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/683
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subject-phosphofructo-2-kinase/fructose 2,6-bisphosphataseen
dc.subjectAgrobacterium tumefaciensen
dc.subjectfructose 2,6-bisphosphateen
dc.subjectRT-PCRen
dc.subjecttransgenic carnationen
dc.subjectDianthus. chinensisen
dc.subjectDianthus caryophyllusen
dc.titleProduction of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNAen
dc.typefolyóiratcikkhu
dc.typearticleen
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