• Neprilysin has an important role in the etiology of some diseases such as cardiovascular diseases. • Neprilysin inhibitors can improve the prognosis of some cardiovascular diseases. • Recent studies found that the combination of neprilysin inhibitors with other drugs such as angiotensin II receptor inhibitor (ARNI) which were used in hypertension, heart failure or diabetes mellitus showed better results than neprilysin inhibitors alone. • Studies on this subject have been greatly developed in recent years, and it is necessary to continue researching this subject to get better results.