Engineering CRISPR Plasmids for Genome Editing in Schizosaccharomyces japonicus

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dc.contributor.advisorPapp, László Attila
dc.contributor.authorDoneva, Marija
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biotechnológiai Intézet
dc.date.accessioned2026-01-07T12:47:47Z
dc.date.available2026-01-07T12:47:47Z
dc.date.created2025-11-20
dc.description.abstractSchizosaccharomyces japonicus represents a dimorphic fission yeast which is able to switch between two forms: a unicellular yeast-like form and a hyphal (filamentous) form. Due to its unique biological features and close evolutionary relationship to humans, S. japonicus has proven to be a valuable model organism for studying cellular processes and mechanisms of human diseases. In this thesis research, our aim was to develop a CRISPR-Cas9 genome editing tool suitable for S. japonicus. To achieve this, pMZ379 plasmid was engineered through the incorporation of the designed sgRNA83 sequence by using a ligation-free PCR-based method. The Cas9 endonuclease successfully introduced double-stranded breaks in the target DNA of the S. japonicus genome and therefore, it was concluded that the CRISPR-Cas9 system is functional in S. japonicus cells. The development of this CRISPR-Cas9 genome editing tool provides a basis for future genetic modifications in S. japonicus.
dc.description.courseBiochemical Engineering
dc.description.degreeBSc/BA
dc.format.extent32
dc.identifier.urihttps://hdl.handle.net/2437/401773
dc.language.isoen
dc.rights.infoHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectSchizosaccharomyces japonicus
dc.subjectCRISPR-Cas9
dc.subjectGenome editing
dc.subject.dspaceBiology
dc.titleEngineering CRISPR Plasmids for Genome Editing in Schizosaccharomyces japonicus
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