Intestinal Epithelial Cells Instruct Myeloid Cell-Mediated T-Cell Responses

A tétel rövid nézete
dc.contributor.advisorRajnavölgyi, Éva
dc.contributor.authorChatterjee, Arunima
dc.contributor.departmentMolekuláris sejt- és immunbiológia doktori iskolahu
dc.contributor.submitterdepDE--Általános Orvostudományi Kar -- Immunológiai Intézet
dc.date.accessioned2015-10-05T16:20:20Z
dc.date.available2015-10-05T16:20:20Z
dc.date.created2015hu_HU
dc.date.defended2015-11-17
dc.description.abstractRetinoids and other derivatives of vitamin A are known to have important functions in regulating differentiation and proliferation of epithelial cells. We have tried to examine the immunological effects of ATRA in colon epithelial cell lines in combination with different pro-inflammatory activators. We chose the two most potent pro-inflammatory cytokines known IL-1β and TNF-α for studying the effect of ATRA. So I used the two cytokines in the presence or absence of ATRA as inducers on Caco2 colon epithelial cell lines for 6hr. We observed that ATRA was responsible for conferring the CD103 cell surface expression patterns on DC and Mf. In case of CX3CR1 surface expression, DC treated with ATRA conditioned CEC supernatant showed an increase in CX3CR1 expression but Mf showed a decrease in CX3CR1 expression with a similar treatment. The fact that ATRA has varied effect on different cells indicates the capability of ATRA to affect the final T-lymphocyte responses. In our in vitro system we observed that ATRA treated CEC supernatant can influence the DC which when co-cultured with CD4+ T lymphocytes, TH17 cells were decreased while for Mf, the number of TH17 cells was significantly increased. Defensins represent an important group of AMP consisting of 16 – 50 amino acids, organized to a structurally conserved compact structure and associated with multiple functions in order to act as a first line of defense mechanism. The three subfamilies of defensins (α, β, θ) differ in their peptide length, location of disulphide bonds, their precursor structures and in the site of expression. Elevated levels β-defensin 3 levels in colonic mucosa of patients with ulcerative colitis suggest their role in the inflammatory response. In this study we have developed a targeted proteomics based method for the determination of defensin levels in cell lysates and cell culture supernatants. Caco2 human epithelial cells were challenged with IL-1β as an inflammatory stimulus and the levels of β-defensin 2 was analyzed in cell lysates and cell culture supernatants. The developed method was validated using qPCR, quantitative ELISA and Western blot. The gene and protein expression levels of β-defensin 2 analyzed were significantly higher in IL-1β treated samples compared to the unstimulated controls. Beside β-defensin 2, the levels of β-defensin 3, β-defensin 4 and α-defensin was examined as well and significantly higher levels of β-defensin 3 could be determined in the supernatants of activated Caco2 cells as well. Our results show that the targeted proteomics method developed here offers an alternative approach for the mass spectrometric analyses of some defensins.hu_HU
dc.description.correctorde
dc.format.extent60hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/216915
dc.language.isoenhu_HU
dc.subjectintestinal epithelial cellshu_HU
dc.subjectchemokines
dc.subjectGM-CSF
dc.subjectproinflammatory cytokines IL-1beta and TNF-alpha
dc.subjectdendritic cells
dc.subjectmacrophages and ILCs in the gut
dc.subjectDefensins, TH17 and Treg
dc.subject.disciplineElméleti orvostudományokhu
dc.subject.sciencefieldOrvostudományokhu
dc.titleIntestinal Epithelial Cells Instruct Myeloid Cell-Mediated T-Cell Responseshu_HU
dc.title.translatedIntestinal Epithelial Cells Instruct Myeloid Cell-Mediated T-Cell Responseshu_HU
Fájlok
Eredeti köteg (ORIGINAL bundle)
Megjelenítve 1 - 3 (Összesen 3)
Nem elérhető
Név:
ARUNIMAChatterjee_Thesis_titkositott.pdf
Méret:
3.28 MB
Formátum:
Adobe Portable Document Format
Leírás:
Dissertation
Letöltés
Nem elérhető
Név:
Arunima_Chatterjee_short_thesis_titkositott.pdf
Méret:
722.1 KB
Formátum:
Adobe Portable Document Format
Leírás:
Short_thesis
Letöltés
Nem elérhető
Név:
ArunimaChatterjee_meghivo.pdf
Méret:
30.93 KB
Formátum:
Adobe Portable Document Format
Leírás:
Meghívó-Invitation
Letöltés
Engedélyek köteg
Megjelenítve 1 - 1 (Összesen 1)
Nem elérhető
Név:
license.txt
Méret:
1.93 KB
Formátum:
Item-specific license agreed upon to submission
Leírás:
Letöltés
Gyűjtemények
Molekuláris Sejt- és Immunbiológia Doktori Iskola