This study investigates the use of toluene diisocyanate (TDI) in the production of polyurethane products, highlighting the benefits of TDI-based polyisocyanate prepolymers for improved chemical and mechanical characteristics and reduced hazards. Matrix-Assisted Laser Desorption/Ionization (MALDI) is utilized to study how TDI interacts with polyethylene glycol (PEG) and polypropylene glycol (PPG) over time. The experimental overview explains TDI reactivity, the roles of PEG and PPG, and the real-time monitoring of reactions using MALDI. In addition, this work focuses on the kinetics of TDI reactions with PEG and PPG, examining variations in peak strengths and the impact of molecular ratios on reaction rates. The findings suggest that MALDI analysis is a valuable tool for assessing TDI-PEG and TDI-PPG rates, enhancing the safety and efficiency of polyurethane production. Furthermore, the study emphasizes the need for a deeper understanding of response rates to improve polyurethane material properties and recommends further research into TDI interactions with other polyols, such as PEG and PPG.