In vitro characterization of HER2-redirected universal CAR T cells

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dc.contributor.advisorSzöőr , Árpád
dc.contributor.advisordeptÁltalános Orvostudományi Kar::Biofizikai és Sejtbiológiai Intézet
dc.contributor.authorLe , Tran Linh Dan
dc.contributor.departmentDE--Általános Orvostudományi Kar
dc.date.accessioned2026-02-26T11:14:07Z
dc.date.available2026-02-26T11:14:07Z
dc.date.created2024-03-07
dc.description.abstractThe thesis provides a comprehensive study on the development and application of Universal CAR T cells (UniCAR T cells) targeted against the HER2 antigen, which is commonly overexpressed in various cancers. The introduction efficiently outlines the context of conventional CAR T cell therapies and their limitations, which sets the stage for the innovative approach of using UniCAR T cells that can be universally applied across different antigens via a modular approach. In this study, UniCAR encoding retro- and lentiviral vectors were produced by replacing the HER2-recognition domain with the mSA2 biotin-binding domain, leading to UniCAR expression in 10-13% of human PBMCs. Coculture assays revealed that biotin-trastuzumab induced HER2-specific UniCAR T cell activation, resulting in IFNγ secretion and targeted lysis of MDA-HER2 tumor cells. Retroviral UniCAR exhibited superior T cell activation in ELISA assays. Novel findings in this topic could revolutionize cancer treatment by offering a highly targeted therapy that reduces off-target effects, potentially leading to improved outcomes and pave the way for innovative approaches in autoimmune disease management.
dc.description.courseáltalános orvos
dc.description.courselangangol
dc.description.degreeegységes, osztatlan
dc.format.extent25
dc.identifier.urihttps://hdl.handle.net/2437/405121
dc.language.isoen
dc.rights.infoHozzáférhető a 2022 decemberi felsőoktatási törvénymódosítás értelmében.
dc.subjectuniversal CAR T cells
dc.subjectcancer
dc.subject.dspaceMedicine
dc.titleIn vitro characterization of HER2-redirected universal CAR T cells
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