Qualitative detection of genetically modified organisms in plant samples

A tétel rövid nézete
dc.contributor.authorUri, Csilla
dc.contributor.authorProkisch, József
dc.contributor.authorSándor, Erzsébet
dc.contributor.authorGyőri, Zoltán
dc.date.accessioned2021-06-28T10:58:56Z
dc.date.available2021-06-28T10:58:56Z
dc.date.issued2007-07-16
dc.description.abstractWe analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl 2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.en
dc.formatapplication/pdf
dc.identifier.citationActa Agraria Debreceniensis, No. 26 (2007) , 309-313
dc.identifier.doihttps://doi.org/10.34101/actaagrar/26/3091
dc.identifier.issn2416-1640
dc.identifier.issue26
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/316614en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/3091
dc.rights.accessOpen Access
dc.subjectGMOen
dc.subjectPCRen
dc.subjectmaizeen
dc.titleQualitative detection of genetically modified organisms in plant samplesen
dc.typefolyóiratcikkhu
dc.typearticleen
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