Optimization of RNA isolation from stone fruits at different ripening stages

A tétel rövid nézete
dc.creatorPfeiffer, P.
dc.creatorHalász, J.
dc.creatorGyörgy, Z.
dc.creatorPedryc, A.
dc.creatorHegedűs, A.
dc.date2009-03-25
dc.date.accessioned2021-06-28T10:00:51Z
dc.date.available2021-06-28T10:00:51Z
dc.descriptionThis study was conducted to select the most appropriate RNA isolation method that can be used successfully in case of stone fruits. The changing pattern of gene expression during the ripening process of stone fruits may elucidate the molecular background of several phenotypical or phytochemical alterations present among different genotypes. Our laboratory aims to study the expression of genes encoding for enzymes that catalyze crucial steps in the flavonoid biosynthesis pathway. RNA isolation from fruit mesocarp is a challanging task due to high levels of sugars and polyphenolics accumulating during fruit development. Therefore, at first, the optimal techniques eligible for RNA isolation from fruit tissues at different ripening stages must be selected. Our study compares three different RNA isolation protocols and describes their potential applicability according to different fruit species and ripening stages.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/IJHS/article/view/820
dc.identifier10.31421/IJHS/15/1-2/820
dc.identifier.urihttp://hdl.handle.net/2437/314529
dc.languageeng
dc.publisherUniversity of Debrecen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/820/818
dc.rightsCopyright (c) 2018 International Journal of Horticultural Science
dc.sourceInternational Journal of Horticultural Science; Vol. 15 No. 1-2 (2009); 101-104.
dc.source2676-931X
dc.source1585-0404
dc.source10.31421/IJHS/15/1-2
dc.subjectapricot
dc.subjectpeach
dc.subjectPrunus
dc.subjectRNA isolation
dc.subjectsour cherry
dc.subjectstone fruits
dc.titleOptimization of RNA isolation from stone fruits at different ripening stages
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
Fájlok
Eredeti köteg (ORIGINAL bundle)
Megjelenítve 1 - 1 (Összesen 1)
Nem elérhető
Név:
pdf.pdf
Méret:
2.01 MB
Formátum:
Adobe Portable Document Format
Letöltés
Gyűjtemények
International Journal of Horticultural Science