Modern methods to determine vitamin E in foods and feeds

A tétel rövid nézete
dc.contributor.authorBara, Camelia
dc.contributor.authorBara, Lucian
dc.contributor.authorBara, Vasile
dc.contributor.authorBara, Ligia
dc.contributor.authorDomuta, Cristian
dc.date.accessioned2021-06-28T11:02:15Z
dc.date.available2021-06-28T11:02:15Z
dc.date.issued2010-10-05
dc.description.abstractHighly specific and sensitive, the proposed fluorometric method for determining α-tocopherol is robust and fairly fast. It has been tested in parallel with a conventional thin layer chromatographic method on foods and feeds. The only necessary cleanup is the usual saponification. The unsaponificable fraction can be extracted with ethyl ether or, preferably, with Extrelut columns. Reagents and their solvents are added to the isooctane solution before each successive reaction and are then eliminated by partition with water. The α-tocopherol (α-T) derivative always remains in isooctane. The first step is nitrosation and elimination of tocopherols and tocotrienols other than α-isomers. α-T is then oxidized to α-tocored (α-TR) with a mixture of sulfuric acid, ferric chloride, and iodine bromide. Α-TR is then condensed to a new reagent: 4,5-dimethylo- phenylenediamine. The phenazine formed is strongly fluorescent. Iodine and bromine add to the double bonds of α- tocotrienol present and quench the fluorescence of its phenazine.en
dc.formatapplication/pdf
dc.identifier.citationActa Agraria Debreceniensis, No. I (2010): Journal Of Agricultural Sciences - Supplement , 53-58
dc.identifier.doihttps://doi.org/10.34101/ACTAAGRAR/I/8374
dc.identifier.issn2416-1640
dc.identifier.issueI
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/317171en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/8374
dc.rights.accessOpen Access
dc.subjecttocopherolen
dc.subjectfooden
dc.subjectfeeden
dc.subjectfluorometryen
dc.titleModern methods to determine vitamin E in foods and feedsen
dc.typefolyóiratcikkhu
dc.typearticleen
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