Evaluation of the optimal temperature for digestive protease function in the hybrid catfish Clarias gariepinus × Heterobranchus longifilis

dc.contributor.authorMartin-Culma, Nazly Yolieth
dc.contributor.authorBársony, Péter
dc.date.accessioned2026-01-14T10:46:20Z
dc.date.available2026-01-14T10:46:20Z
dc.date.issued2025-12-02
dc.description.abstract(1) Background: Water temperature is a key determinant of fish digestion. In vitro methods provide a reliable approach to assess enzyme activity, which is especially important in carnivorous catfish with protein-rich diets. Although the hybrid catfish Clarias gariepinus × Heterobranchus longifilis is widely farmed in Hungary, its optimal temperature for maximizing digestibility remains under investigation.; (2) Methods: Fish were reared at 23 ºC, 25 ºC, and 27 ºC, and stomach and proximal intestine content samples were collected, and it was determined the protease activity (3) Results: No statistically significant differences were observed in enzyme efficiency, crude protease activity (p > 0.05). Despite not significant differences, the stomach and intestinal, protease activity tended to be higher at 25 °C (3.41 ± 0.16 U/mL), and, while in the intestines was higher at 27 °C (2.03 ± 0.27 U/mL). Stomach activity was higher at 27 ºC, at 27 °C (2.03 ± 0.27 U/mL, respectively. (4) Conclusions: Stomach protease activity tended to be higher at 25 °C, while intestinal activity was highest at 25–27 °C. These results may indicate a better starting digestion that supports feed digestion.    en
dc.formatapplication/pdf
dc.identifier.citationActa Agraria Debreceniensis, No. 2 (2025) , 65-70
dc.identifier.doihttps://doi.org/10.34101/actaagrar/2/16195
dc.identifier.issn2416-1640
dc.identifier.issue2
dc.identifier.jatitleActa agrar. Debr.
dc.identifier.jtitleActa Agraria Debreceniensis
dc.identifier.urihttps://hdl.handle.net/2437/402036en
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/16195
dc.rights.accessOpen Access
dc.rights.ownerby the Author(s)
dc.subjectin vitroen
dc.subjectfish digestionen
dc.subjectprotease activityen
dc.subjecttemperatureen
dc.titleEvaluation of the optimal temperature for digestive protease function in the hybrid catfish Clarias gariepinus × Heterobranchus longifilisen
dc.typefolyóiratcikkhu
dc.typearticleen
dc.type.detailedidegen nyelvű folyóiratközlemény hazai lapbanhu
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