In this study, Lactobacillus plantarum ATCC 14917 strain was investigated to reveal the extracellular metabolites produced during the fermentation of MRS culture by this strain. In MRS medium glucose is the carbon cource. We divided metabolites to seven compound classes based on functional groups: amino acids, nitrogen-containing compounds (excluding amino acids and nucleotides), carboxylic acids, alcohols, alkaloids, aromatic compounds, and alkanes/alkenes. Results show increased production of bioactive short-chain fatty acids (SCFAs), along with essential amino acids and alkaloids, under glucose-rich conditions. These metabolites support gut health, immunity, and microbiota balance. Our study further demonstrated the pathways through which these metabolites are produced and released by Lactobacillus plantarum, providing deeper insight into its metabolic mechanisms and possible probiotic potential.