New simple and efficient method of DNA isolation from pear leaves rich in polyphenolic compounds

A tétel rövid nézete
dc.contributor.authorBokszczanin, K.
dc.contributor.authorPrzybyla, A. A.
dc.date.accessioned2021-06-28T09:59:55Z
dc.date.available2021-06-28T09:59:55Z
dc.date.issued2006-09-26
dc.description.abstractThis study aimed to establish a new protocol for DNA isolation from the Pyrus genus to get high quality DNA that is suitable for the generation of molecular markers, such as RAPD and AFLP. This method is based on modified CTAB extraction procedure (Aldrich & Cullis, 1993). For isolation of high-quality DNA we used copper (II) acetate treatment that enabled fixation and removing of tannins, present in abundance in Pyrus. DNA yield from this procedure is high. DNA is completely digestible with restriction endonucleases and amplifiable in the PCR, indicating freedom from common contaminating polyphenolic compounds.en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 12 No. 4 (2006) , 21-24.
dc.identifier.doihttps://doi.org/10.31421/IJHS/12/4/673
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue4
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314410en
dc.identifier.volume12
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/673
dc.rights.accessOpen Access
dc.rights.ownerInternational Journal of Horticultural Science
dc.subjectpear DNA isolationen
dc.subjectpolyphenolic compoundsen
dc.subjecttanninsen
dc.subjectcopper (II) acetateen
dc.subjectPyrusen
dc.titleNew simple and efficient method of DNA isolation from pear leaves rich in polyphenolic compoundsen
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)