Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS

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dc.creatorHorváth, Brigitta
dc.creatorPeles, Ferenc
dc.creatorBereczki, László
dc.creatorSzéll, András
dc.creatorSipos, Rita
dc.creatorErős, Ágnes
dc.creatorLovász, Csaba
dc.creatorMicsinai, Adrienn
dc.date2019-05-23
dc.date.accessioned2021-06-28T10:55:15Z
dc.date.available2021-06-28T10:55:15Z
dc.descriptionCoagulase-positive staphylococci include 3 species, Staphylococcus aureus, S. hyicus and S. intermedius. Of these three species, S. aureus is the most well-known human pathogen. S. aureus is part of the human and animal normal microbiota, however, it is capable of producing several staphylococcal enterotoxins (SEs) that cause intoxication symptoms of varying intensity in humans after consuming contaminated food. Selective media which are used for the determination of coagulase-positive staphylococci from foods are not able to identify isolates at a species. With the MALDI-TOF MS technique, we can identify S. aureus cheaper and faster than by using molecular methods. This paper describes the results of the study of the presence of coagulase-positive staphylococci and S. aureus in many food products, and the application of three sample preparation methods: direct sample preparation, formic acid suspension and ethanol extraction.
dc.formatapplication/pdf
dc.identifierhttps://ojs.lib.unideb.hu/actaagrar/article/view/2362
dc.identifier10.34101/actaagrar/1/2362
dc.identifier.urihttp://hdl.handle.net/2437/316027
dc.languageeng
dc.publisherFaculty of Agricultural and Food Sciences and Enviromental Management of the Debrecen University, Debrecen.
dc.relationhttps://ojs.lib.unideb.hu/actaagrar/article/view/2362/2485
dc.sourceActa Agraria Debreceniensis; No. 1 (2019); 9-14
dc.source2416-1640
dc.source1587-1282
dc.subjectCoagulase-positive staphylococci
dc.subjectMALDI-TOF MS
dc.subjectsample preparation
dc.subjectfoodborne pathogen
dc.subjectS. aureus
dc.titleComparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Article
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Acta Agraria Debreceniensis