Transformation of fission yeast ace2 deletion mutant

A tétel rövid nézete
dc.contributor.advisorBatta, Gyula
dc.contributor.authorArkhatbayeva, Darina
dc.contributor.departmentDE--Természettudományi és Technológiai Kar--Biotechnológiai Intézethu_HU
dc.date.accessioned2021-11-15T09:47:45Z
dc.date.available2021-11-15T09:47:45Z
dc.date.created2021-11-04
dc.description.abstractThe aim of the thesis work was to perform transformation of ace2 deletion mutant with different length ace2-expressing plasmids to demonstrate whether the original phenotype of wild type Sch. pombe is restored. Ace2 gene is crucial, since it is responsible for complete daughter cells separation during cell division of fission yeast. After transformation, only few colonies have grown. Several techniques, including PCR reactions and gel electrophoresis, were performed in order to check the ace2 and plasmids presence. Under certain assumptions, it was concluded that low transformation success rate was due to cell wall thickening in mutant and too long sequences missing from the ace2 plasmids.hu_HU
dc.description.correctorhbk
dc.description.courseBiochemical Engineeringhu_HU
dc.description.degreeBSc/BAhu_HU
dc.format.extent36hu_HU
dc.identifier.urihttp://hdl.handle.net/2437/324621
dc.language.isoenhu_HU
dc.subjectAce 2hu_HU
dc.subjectFission yeasthu_HU
dc.subject.dspaceDEENK Témalista::Biológiai tudományokhu_HU
dc.titleTransformation of fission yeast ace2 deletion mutanthu_HU
Fájlok
Gyűjtemények
Theses (Faculty of Science and Technology)