Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.

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dc.contributor.authorSzőke, E.
dc.contributor.authorBálványos, I.
dc.contributor.authorKursinszki, L.
dc.contributor.authorKrajewska, A.
dc.contributor.authorMészáros, A.
dc.contributor.authorNeszmélyi, A.
dc.date.accessioned2021-06-28T09:57:59Z
dc.date.available2021-06-28T09:57:59Z
dc.date.issued2001-06-06
dc.description.abstractThe investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.  en
dc.formatapplication/pdf
dc.identifier.citationInternational Journal of Horticultural Science, Vol. 7 No. 2 (2001) , 65-71.
dc.identifier.doihttps://doi.org/10.31421/IJHS/7/2/269
dc.identifier.eissn2676-931X
dc.identifier.issn1585-0404
dc.identifier.issue2
dc.identifier.jatitleInt. j. hortic. sci.
dc.identifier.jtitleInternational Journal of Horticultural Science
dc.identifier.urihttps://hdl.handle.net/2437/314105en
dc.identifier.volume7
dc.languageen
dc.relationhttps://ojs.lib.unideb.hu/IJHS/article/view/269
dc.rights.accessOpen Access
dc.subjectalkaloiden
dc.subjectamino aciden
dc.subjectgrowth regulatoren
dc.subjecthairy rooten
dc.subjectLobelia inflataen
dc.subjectlobelineen
dc.titleStudies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.en
dc.typefolyóiratcikkhu
dc.typearticleen
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International Journal of Horticultural Science (DE-journals)